Micromirror structured illumination microscope for high-speed in vivo drosophila brain imaging.
Opt Express
; 22(2): 1243-56, 2014 Jan 27.
Article
en En
| MEDLINE
| ID: mdl-24515130
Genetic tools and especially genetically encoded fluorescent reporters have given a special place to optical microscopy in drosophila neurobiology research. In order to monitor neural networks activity, high speed and sensitive techniques, with high spatial resolution are required. Structured illumination microscopies are wide-field approaches with optical sectioning ability. Despite the large progress made with the introduction of the HiLo principle, they did not meet the criteria of speed and/or spatial resolution for drosophila brain imaging. We report on a new implementation that took advantage of micromirror matrix technology to structure the illumination. Thus, we showed that the developed instrument exhibits a spatial resolution close to that of confocal microscopy but it can record physiological responses with a speed improved by more than an order a magnitude.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Encéfalo
/
Iluminación
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Aumento de la Imagen
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Drosophila
/
Lentes
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Microscopía Fluorescente
Límite:
Animals
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Estados Unidos