Your browser doesn't support javascript.
loading
Morphogenetic study on the maturation of osteoblastic cell as induced by inorganic polyphosphate.
Tsutsumi, Kaori; Saito, Nagahito; Kawazoe, Yumi; Ooi, Hong-Kean; Shiba, Toshikazu.
Afiliación
  • Tsutsumi K; Department of Biological Sciences and Engineering, Faculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
  • Saito N; Department of Internal Medicine, Gastroenterology & Hematology Section, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan.
  • Kawazoe Y; Regenetiss Inc., Kunitachi, Tokyo, Japan.
  • Ooi HK; Department of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara-Shi, Kanagawa, Japan.
  • Shiba T; Regenetiss Inc., Kunitachi, Tokyo, Japan ; Laboratory for Polyphosphate Research, The Kitasato Institute, Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
PLoS One ; 9(2): e86834, 2014.
Article en En | MEDLINE | ID: mdl-24498284
Since inorganic polyphosphates [poly(P)] have an activity to induce bone differenciation in vitro and in vivo, we examined an effect of poly(P) on organelle by light microscopy and electron microscopy in Murine MC3T3-E1 osteoblastic cells. The MC3T3-E1 cells were ultrastructurally observed to possess morphological characteristics of osteoblasts. Cells cultured with poly(P) were strongly stained with an anti-collagen type I antibody but not in those cultured without poly(P). Ultrastructural analysis of cells cultured with poly(P) revealed a well-developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria and many coated pits. Since MC3T3-E1 cells can be transformed from a resting phase to an active blastic cell phase after supplementation with poly(P), it implies that poly(P) can be an effective material for bone regeneration.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Osteogénesis / Polifosfatos / Diferenciación Celular Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Osteogénesis / Polifosfatos / Diferenciación Celular Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos