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Production of rhesus monkey cloned embryos expressing monomeric red fluorescent protein by interspecies somatic cell nuclear transfer.
Zhu, Hai-Ying; Kang, Jin-Dan; Li, Suo; Jin, Jun-Xue; Hong, Yu; Jin, Long; Guo, Qing; Gao, Qing-Shan; Yan, Chang-Guo; Yin, Xi-Jun.
Afiliación
  • Zhu HY; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Kang JD; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Li S; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Jin JX; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Hong Y; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Jin L; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Guo Q; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Gao QS; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Yan CG; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China.
  • Yin XJ; Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, PR China. Electronic address: yinxj33@msn.com.
Biochem Biophys Res Commun ; 444(4): 638-43, 2014 Feb 21.
Article en En | MEDLINE | ID: mdl-24491539
Interspecies somatic cell nuclear transfer (iSCNT) is a promising method to clone endangered animals from which oocytes are difficult to obtain. Monomeric red fluorescent protein 1 (mRFP1) is an excellent selection marker for transgenically modified cloned embryos during somatic cell nuclear transfer (SCNT). In this study, mRFP-expressing rhesus monkey cells or porcine cells were transferred into enucleated porcine oocytes to generate iSCNT and SCNT embryos, respectively. The development of these embryos was studied in vitro. The percentage of embryos that underwent cleavage did not significantly differ between iSCNT and SCNT embryos (P>0.05; 71.53% vs. 80.30%). However, significantly fewer iSCNT embryos than SCNT embryos reached the blastocyst stage (2.04% vs. 10.19%, P<0.05). Valproic acid was used in an attempt to increase the percentage of iSCNT embryos that developed to the blastocyst stage. However, the percentages of embryos that underwent cleavage and reached the blastocyst stage were similar between untreated iSCNT embryos and iSCNT embryos treated with 2mM valproic acid for 24h (72.12% vs. 70.83% and 2.67% vs. 2.35%, respectively). These data suggest that porcine-rhesus monkey interspecies embryos can be generated that efficiently express mRFP1. However, a significantly lower proportion of iSCNT embryos than SCNT embryos reach the blastocyst stage. Valproic acid does not increase the percentage of porcine-rhesus monkey iSCNT embryos that reach the blastocyst stage. The mechanisms underling nuclear reprogramming and epigenetic modifications in iSCNT need to be investigated further.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clonación de Organismos / Proteínas Luminiscentes / Macaca mulatta Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clonación de Organismos / Proteínas Luminiscentes / Macaca mulatta Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos