Specific immunoradiometric assay of insulin-like growth factor I with use of monoclonal antibodies.
Clin Chem
; 33(11): 2019-23, 1987 Nov.
Article
en En
| MEDLINE
| ID: mdl-2445506
We identified two monoclonal antibodies that bind spatially distinct epitopes on insulin-like growth factor I (IGF-I). Using these two antibodies, we developed a simultaneous, two-site immunoradiometric assay (IRMA) specific for IGF-I. This IRMA has no detectable cross reactivity with insulin, proinsulin, prolactin, or somatotropin, and less than 2% crossreactivity with IGF-II. The assay response varies linearly with IGF-I concentrations of 0-800 micrograms/L in serum; the detection limit is about 10 micrograms/L. A comparison of 26 IGF-I serum values from the IRMA and from a previously reported IGF-I specific RIA gave a correlation coefficient of 0.96 with no substantial bias (slope = 1.10). IGF-I values for serum, as an aid in assessing growth abnormalities, are easily (only three pipetting steps) obtained in less than 4 h.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Somatomedinas
/
Factor I del Crecimiento Similar a la Insulina
/
Inmunoensayo
/
Radioisótopos de Yodo
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
1987
Tipo del documento:
Article
Pais de publicación:
Reino Unido