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A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides.
Prober, J M; Trainor, G L; Dam, R J; Hobbs, F W; Robertson, C W; Zagursky, R J; Cocuzza, A J; Jensen, M A; Baumeister, K.
Afiliación
  • Prober JM; Engineering Physics Laboratory, E.I. du Pont de Nemours & Company (Inc.), Wilmington, DE 19898.
Science ; 238(4825): 336-41, 1987 Oct 16.
Article en En | MEDLINE | ID: mdl-2443975
A DNA sequencing system based on the use of a novel set of four chain-terminating dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye distinguished by its fluorescent emission is described. Avian myeloblastosis virus reverse transcriptase is used in a modified dideoxy DNA sequencing protocol to produce a complete set of fluorescence-tagged fragments in one reaction mixture. These DNA fragments are resolved by polyacrylamide gel electrophoresis in one sequencing lane and are identified by a fluorescence detection system specifically matched to the emission characteristics of this dye set. A scanning system allows multiple samples to be run simultaneously and computer-based automatic base sequence identifications to be made. The sequence analysis of M13 phage DNA made with this system is described.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Secuencia de Bases / Desoxirribonucleótidos / Colorantes Fluorescentes Tipo de estudio: Prognostic_studies Idioma: En Revista: Science Año: 1987 Tipo del documento: Article Pais de publicación: Estados Unidos
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Secuencia de Bases / Desoxirribonucleótidos / Colorantes Fluorescentes Tipo de estudio: Prognostic_studies Idioma: En Revista: Science Año: 1987 Tipo del documento: Article Pais de publicación: Estados Unidos