Preparation of highly enriched photosystem II membrane vesicles by a non-detergent method.
Photosynth Res
; 20(3): 249-59, 1989 Jun.
Article
en En
| MEDLINE
| ID: mdl-24424437
An improved, non-detergent, method for preparative isolation of PS II membrane vesicles from spinach chloroplasts is presented. Thylakoids (chlorophyll (Chl) a/b ratio 2.8, Chl/P700 435) were fractionated by Yeda press treatment and aqueous two-phase partition to yield inside-out vesicles (1) (chl a/b 2.2, chl/P700 700). These vesicles were subjected a sonication - phase partitioning procedure; steps of sonication of inside-out vesicles, while still present in a dextran-polyethylene glycol two-phase system were alternated by phase partition. These steps selectively removed P700-containing membrane fragments from the inside-out vesicles and yielded a membrane fraction with improved PS II purity (Chl a/b ratio 1.9, Chl/P700 1500) and retained oxygen evolving capacity (295 µmol O2 mg Chl(-1) h(-1)).
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Photosynth Res
Asunto de la revista:
METABOLISMO
Año:
1989
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Países Bajos