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Combined evaluation of commonly used techniques, including PCR, for diagnosis of mouse fur mites.
Karlsson, Eleanor M; Pearson, Laura M; Kuzma, Kristen M; Burkholder, Tanya H.
Afiliación
  • Karlsson EM; Division of Veterinary Resources, Office of Research Services, National Institutes of Health, Bethesda, Maryland, USA. ellie.m.karlsson@gmail.com.
  • Pearson LM; Division of Veterinary Resources, Office of Research Services, National Institutes of Health, Bethesda, Maryland, USA.
  • Kuzma KM; Division of Veterinary Resources, Office of Research Services, National Institutes of Health, Bethesda, Maryland, USA.
  • Burkholder TH; Division of Veterinary Resources, Office of Research Services, National Institutes of Health, Bethesda, Maryland, USA.
J Am Assoc Lab Anim Sci ; 53(1): 69-73, 2014 Jan.
Article en En | MEDLINE | ID: mdl-24411782
Our study evaluated and compared the false-negative rates (FNR) of a wide array of fur-mite diagnostic tests, including 2 postmortem tests (pelt exam and sticky paper) and 3 antemortem tests (adhesive tape, fur pluck, and PCR). Past publications examining fur-mite diagnostic techniques primarily used paired comparisons, evaluating tests by their level of agreement with only one other test. However, different combinations or pairs of diagnostics are used in the different studies, making the results of these comparisons difficult to interpret across all available diagnostics. In the current study, mice from a conventionally maintained colony endemic for Myobia musculi were identified as positive based on at least one positive diagnostic test. From this pool of positive animals, the FNR of all tests were quantified. The PCR assay and the pelt exam performed the best, with 0% and 2% FNR respectively, whereas tape, fur-pluck, and sticky-paper tests showed 24%, 26%, and 36% FNR, respectively. Our study shows that for mice in a colony naturally infested with Myobia musculi, PCR testing can be used for reliable antemortem detection, and pelt exam performed by experienced examiners is reliable for postmortem detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Roedores / Reacción en Cadena de la Polimerasa / Pruebas Diagnósticas de Rutina / Cabello / Infestaciones por Ácaros / Ácaros Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Am Assoc Lab Anim Sci Asunto de la revista: MEDICINA VETERINARIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Roedores / Reacción en Cadena de la Polimerasa / Pruebas Diagnósticas de Rutina / Cabello / Infestaciones por Ácaros / Ácaros Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Am Assoc Lab Anim Sci Asunto de la revista: MEDICINA VETERINARIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos