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Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration.
Wagner, Darcy E; Bonenfant, Nicholas R; Sokocevic, Dino; DeSarno, Michael J; Borg, Zachary D; Parsons, Charles S; Brooks, Elice M; Platz, Joseph J; Khalpey, Zain I; Hoganson, David M; Deng, Bin; Lam, Ying W; Oldinski, Rachael A; Ashikaga, Takamaru; Weiss, Daniel J.
Afiliación
  • Wagner DE; University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: Darcy.wagner@med.uvm.edu.
  • Bonenfant NR; University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: Nicholas.Bonenfant@med.uvm.edu.
  • Sokocevic D; University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: Dino.Sokocevic@med.uvm.edu.
  • DeSarno MJ; University of Vermont, Department of Medical Biostatistics, 27 Hills Building, Burlington, VT 05405, USA. Electronic address: mdesarno@uvm.edu.
  • Borg ZD; University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: zachary.borg@med.uvm.edu.
  • Parsons CS; University of Vermont, Department of Surgery, Fletcher House 301, Burlington, VT 05405, USA. Electronic address: charles.parsons@vtmednet.org.
  • Brooks EM; University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: elice.brooks@med.uvm.edu.
  • Platz JJ; University of Vermont, Department of Surgery, Fletcher House 301, Burlington, VT 05405, USA. Electronic address: Joseph.Platz@med.uvm.edu.
  • Khalpey ZI; University of Arizona, Department of Surgery, 1501 North Campbell Avenue, Tucson, AZ 85724, USA. Electronic address: zkhalpey@surgery.arizona.edu.
  • Hoganson DM; Washington University in St. Louis, Department of Surgery, 1 Barnes Jewish Plaza, 3108 Queeny Tower, St. Louis, MO 63110, USA. Electronic address: hogansondm@wudosis.wustl.edu.
  • Deng B; University of Vermont, Department of Biology, 311 Marsh Life Sciences, Burlington, VT 05405, USA. Electronic address: bdeng@uvm.edu.
  • Lam YW; University of Vermont, Department of Biology, 311 Marsh Life Sciences, Burlington, VT 05405, USA. Electronic address: Ying-Wai.Lam@uvm.edu.
  • Oldinski RA; University of Vermont, College of Engineering and Mathematics, 301 Votey Hall, 33 Colchester Ave, Burlington, VT 05405, USA. Electronic address: oldinski@uvm.edu.
  • Ashikaga T; University of Vermont, Department of Medical Biostatistics, 27 Hills Building, Burlington, VT 05405, USA. Electronic address: Takamaru.Ashikaga@uvm.edu.
  • Weiss DJ; University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: dweiss@uvm.edu.
Biomaterials ; 35(9): 2664-79, 2014 Mar.
Article en En | MEDLINE | ID: mdl-24411675
Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (∼ 1-3 cm(3)) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Ingeniería de Tejidos / Andamios del Tejido / Pulmón / Enfermedades Pulmonares Límite: Animals / Humans Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Ingeniería de Tejidos / Andamios del Tejido / Pulmón / Enfermedades Pulmonares Límite: Animals / Humans Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos