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A universal gene carrier platform for treatment of human prostatic carcinoma by p53 transfection.
Han, Lu; Zhao, Jing; Liu, Juan; Duan, Xiang-Lin; Li, Lu-Hai; Wei, Xian-Fu; Wei, Yen; Liang, Xing-Jie.
Afiliación
  • Han L; Beijing Printed Electronics Engineering Technology Research Center, Beijing Institute of Graphic Communication, Beijing 102600, PR China; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China.
  • Zhao J; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang 050016, PR China.
  • Liu J; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China.
  • Duan XL; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang 050016, PR China.
  • Li LH; Beijing Printed Electronics Engineering Technology Research Center, Beijing Institute of Graphic Communication, Beijing 102600, PR China.
  • Wei XF; Beijing Printed Electronics Engineering Technology Research Center, Beijing Institute of Graphic Communication, Beijing 102600, PR China.
  • Wei Y; Beijing Printed Electronics Engineering Technology Research Center, Beijing Institute of Graphic Communication, Beijing 102600, PR China; Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, P
  • Liang XJ; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China. Electronic address: liangxj@nanoctr.cn.
Biomaterials ; 35(9): 3110-20, 2014 Mar.
Article en En | MEDLINE | ID: mdl-24411335
Our previous work showed that a charge-reversal layer-by-layer nanosystem, PEI/PAH-Cit/AuNP-CS, effectively facilitates cellular uptake of siRNA and enhances the silencing efficacy of MDR1 siRNA. Here, the plasmid loading capacity of this vehicle was examined using EGFP-N1, and the plasmid release profile was determined in response to pH changes. The cytotoxicity of the EGFP-N1/PEI/PAH-Cit/AuNP-CS complex against HeLa and 293T cells was almost negligible. PEI/PAH-Cit/AuNP-CS efficaciously delivered the plasmids EGFP-N1 (encoding green fluorescent protein) and pGL3.0 (encoding luciferase) into 293T and HeLa cells, thus verifying the universality of PEI/PAH-Cit/AuNP-CS as a gene carrier. The results of an inverted fluorescence microscopy, flow cytometry (FCM) and western blotting methods demonstrated that PC-3 prostate cancer cells treated with EGFP-p53/PEI/PAH-Cit/AuNP-CS expressed higher levels of GFP than cells treated with EGFP-p53/PEI. Furthermore, PC-3 cells treated with EGFP-p53/PEI/PAH-Cit/AuNP-CS showed reduced cellular viability and increased nuclear fragmentation, consistent with elevated p53 expression. Propidium iodide (PI) flow cytometric assays were conducted to demonstrate that EGFP-p53/PEI/PAH-Cit/AuNP-CS elevated the level of apoptosis in PC-3 cells. Western blotting and caspase activation studies revealed that EGFP-p53/PEI/PAH-Cit/AuNP-CS complexes may induce PC-3 apoptosis via the mitochondria-mediated signaling pathway by up-regulation of Bax, down-regulation of Bcl-2, and activation of caspase-3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Transfección / Proteína p53 Supresora de Tumor Límite: Humans / Male Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Transfección / Proteína p53 Supresora de Tumor Límite: Humans / Male Idioma: En Revista: Biomaterials Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos