Identification of pregnancy-associated glycoproteins and alpha-fetoprotein in fallow deer (Dama dama) placenta.
Acta Vet Scand
; 56: 4, 2014 Jan 13.
Article
en En
| MEDLINE
| ID: mdl-24410890
BACKGROUND: This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from fallow deer (Dama dama) pregnant females. Proteins issued from FCT and MCT were submitted to affinity chromatographies by using Vicia villosa agarose (VVA) or anti-bovine PAG-2 (R#438) coupled to Sepharose 4B gel. Finally, they were characterized by SDS-PAGE and N-terminal microsequencing. RESULTS: Four distinct fallow deer PAG (fdPAG) sequences were identified and submitted to Swiss-Prot database. Comparison of fdPAG with PAG sequences identified in other ruminant species exhibited 64 to 83% identity. Additionally, alpha-fetoprotein was identified in fetal and maternal tissues. CONCLUSION: Our results demonstrate the efficacy of VVA and bovine PAG-2 affinity chromatographies for the isolation of PAG molecules expressed in deer placenta. This is the first report giving four specific amino acid sequences of PAG isolated from feto-maternal junction (FCT and MCT) in the Cervidae family.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Placenta
/
Proteínas Gestacionales
/
Ciervos
/
Glicoproteínas
/
Alfa-Fetoproteínas
/
Cromatografía de Afinidad
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Risk_factors_studies
Límite:
Animals
/
Pregnancy
País/Región como asunto:
Europa
Idioma:
En
Revista:
Acta Vet Scand
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Reino Unido