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Production of NY-ESO-1 peptide/DRB1*08:03 tetramers and ex vivo detection of CD4 T-cell responses in vaccinated cancer patients.
Mizote, Yu; Uenaka, Akiko; Isobe, Midori; Wada, Hisashi; Kakimi, Kazuhiro; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Oka, Mikio; Nakayama, Eiichi.
Afiliación
  • Mizote Y; Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Department of Respiratory Medicine, Kawasaki Medical School, Okayama, Japan.
  • Uenaka A; Faculty of Health and Welfare, Kawasaki University of Medical Welfare, Kurashiki 701-0193, Okayama, Japan.
  • Isobe M; Department of Respiratory Medicine, Kawasaki Medical School, Okayama, Japan.
  • Wada H; Department of Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.
  • Kakimi K; Department of Immunotherapeutics, University of Tokyo Hospital, Tokyo, Japan.
  • Saika T; Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Kita S; Medical & Biological Laboratories, Nagano, Japan.
  • Koide Y; Medical & Biological Laboratories, Nagano, Japan.
  • Oka M; Department of Respiratory Medicine, Kawasaki Medical School, Okayama, Japan.
  • Nakayama E; Faculty of Health and Welfare, Kawasaki University of Medical Welfare, Kurashiki 701-0193, Okayama, Japan. Electronic address: nakayama@mw.kawasaki-m.ac.jp.
Vaccine ; 32(8): 957-64, 2014 Feb 12.
Article en En | MEDLINE | ID: mdl-24397899
We established CD4 T-cell clones, Mz-1B7, and Ue-21, which recognized the NY-ESO-1 121-138 peptide from peripheral blood mononuclear cells (PBMCs) of an esophageal cancer patient, E-2, immunized with an NY-ESO-1 protein and determined the NY-ESO-1 minimal epitopes. Minimal peptides recognized by Mz-1B7 and Ue-21 were NY-ESO-1 125-134 and 124-134, respectively, both in restriction to DRB1*08:03. Using a longer peptide, 122-135, and five other related peptides, including either of the minimal epitopes recognized by the CD4 T-cell clones, we investigated the free peptide/DR recognition on autologous EBV-B cells as APC and peptide/DR tetramer binding. The results showed a discrepancy between them. The tetramers with several peptides recognized by either Mz-1B7 or the Ue-21 CD4 T-cell clone did not bind to the respective clone. On the other hand, unexpected binding of the tetramer with the peptide not recognized by CD4 T-cells was observed. The clone Mz-1B7 did not recognize the free peptide 122-135 on APC, but the peptide 122-135/DRB1*08:03 tetramer bound to the TCR on those cells. The failure of tetramer production and the unexpected tetramer binding could be due to a subtly modified structure of the peptide/DR tetramer from the structure of the free peptide/DR molecule. We also demonstrated that the NY-ESO-1 123-135/DRB1*08:03 tetramer detected ex vivo CD4 T-cell responses in PBMCs from patients after NY-ESO-1 vaccination in immunomonitoring.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Vacunas contra el Cáncer / Cadenas beta de HLA-DR / Proteínas de la Membrana / Antígenos de Neoplasias Tipo de estudio: Diagnostic_studies Límite: Humans / Male Idioma: En Revista: Vaccine Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Vacunas contra el Cáncer / Cadenas beta de HLA-DR / Proteínas de la Membrana / Antígenos de Neoplasias Tipo de estudio: Diagnostic_studies Límite: Humans / Male Idioma: En Revista: Vaccine Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos