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Phytochemical and antibacterial investigations of the extracts and fractions from the stem bark of Hymenaea stigonocarpa Mart. ex Hayne and effect on ultrastructure of Staphylococcus aureus induced by hydroalcoholic extract.
Dimech, Gustavo Santiago; Soares, Luiz Alberto Lira; Ferreira, Magda Assunção; de Oliveira, Anne Gabrielle Vasconcelos; Carvalho, Maria da Conceição; Ximenes, Eulália Azevedo.
Afiliación
  • Dimech GS; Laboratório de Fisiologia e Bioquímica de Microorganismos, Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
  • Soares LA; Laboratório de Farmacognosia, Departamento de Farmácia Universidade Federal de Pernambuco, Centro de Ciências da Saúde, 50670-901 Recife, PE, Brazil.
  • Ferreira MA; Laboratório de Farmacognosia, Departamento de Farmácia Universidade Federal de Pernambuco, Centro de Ciências da Saúde, 50670-901 Recife, PE, Brazil.
  • de Oliveira AG; Laboratório de Biologia Celular e Ultraestrutura, Centro de Tecnologias Estratégicas do Nordeste (CETENE), 50670-901 Recife, PE, Brazil.
  • Carvalho Mda C; Laboratório de Biologia Celular e Ultraestrutura, Centro de Tecnologias Estratégicas do Nordeste (CETENE), 50670-901 Recife, PE, Brazil.
  • Ximenes EA; Laboratório de Fisiologia e Bioquímica de Microorganismos, Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
ScientificWorldJournal ; 2013: 862763, 2013.
Article en En | MEDLINE | ID: mdl-24396311
The aim of this study was to investigate the antimicrobial activity of different extracts and fractions obtained from Hymenaea stigonocarpa stem barks. The cyclohexanic, ethyl acetate, ethanol, aqueous, and hydroalcoholic extracts were obtained by maceration. The hydroalcoholic extract was partitioned, which resulted in the ethyl acetate and aqueous fractions. All extracts and fractions were subjected to phytochemical screening and evaluation of total phenol and tannin contents. An HPLC-DAD and ultrastructural alterations analysis were performed. Terpenes and coumarins were detected in the cyclohexanic extract. Flavonoids and condensed tannins were present in the other extracts and fractions. The extracts with the highest contents of tannins, ethanol (EE), hydroalcoholic (HE), and aqueous fraction (AF) showed also the highest antimicrobial activity. The MIC values ranged from 64 to 526 µg/mL. The chromatographic fingerprints suggest the presence of astilbin and other flavonoids in EE and HE. Presence of the thick cell wall, undulating outer layer, abnormal septa, and leakage of the cytoplasmic contents and absence of cell wall and cell lyses were the main alterations observed on Staphylococcus aureus ATCC 33591 after treatment with the Hymenaea stigonocarpa hydroalcoholic extract. The presence of phenolic compounds like flavonoids and tannins is possibly the reason for the antimicrobial activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Corteza de la Planta / Hymenaea / Staphylococcus aureus Resistente a Meticilina / Antibacterianos Idioma: En Revista: ScientificWorldJournal Asunto de la revista: MEDICINA Año: 2013 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Corteza de la Planta / Hymenaea / Staphylococcus aureus Resistente a Meticilina / Antibacterianos Idioma: En Revista: ScientificWorldJournal Asunto de la revista: MEDICINA Año: 2013 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos