Continuous passages accelerate the reprogramming of mouse induced pluripotent stem cells.
Cell Reprogram
; 16(1): 77-83, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24387163
Induced pluripotent stem cells (iPSCs) are usually generated by reprogramming somatic cells through transduction with a transcription factor cocktail. However, the low efficiency of this procedure has kept iPSCs away from the study of the clinical application of stem cell biology. Our research shows that continuous passage increases the efficiency of reprogramming. Compared with conventional method of establishment of iPSCs, more embryonic stem cell (ESC)-like clones are generated by continuous passage during early reprogramming. These inchoate clones, indistinguishable from genuine ESC clones, are closer to fully reprogrammed cells compared with those derived from classical iPSC induction, which increased the expression of pluripotent gene markers and the levels of demethylation of Oct4 and Nanog. These results suggested that full reprogramming is a gradual process that does not merely end at the point of the activation of endogenous pluripotency-associated genes. Continuous passage could increase the pluripotency of induced cells and accelerate the process of reprogramming by epigenetic modification. In brief, we have provided an advanced strategy to accelerate the reprogramming and generate more nearly fully reprogrammed iPSCs efficiently and rapidly.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Antígenos de Diferenciación
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Desdiferenciación Celular
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Células Madre Pluripotentes Inducidas
Límite:
Animals
Idioma:
En
Revista:
Cell Reprogram
Año:
2014
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos