Nucleotide-dependent displacement and dynamics of the &#945;-1 helix in kinesin revealed by site-directed spin labeling EPR.

Yasuda, Satoshi; Yanagi, Takanori; Yamada, Masafumi D; Ueki, Shoji; Maruta, Shinsaku; Inoue, Akio; Arata, Toshiaki

Nucleotide-dependent displacement and dynamics of the α-1 helix in kinesin revealed by site-directed spin labeling EPR.

Yasuda, Satoshi; Yanagi, Takanori; Yamada, Masafumi D; Ueki, Shoji; Maruta, Shinsaku; Inoue, Akio; Arata, Toshiaki.

Afiliación

Yasuda S; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Yanagi T; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Yamada MD; Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.
Ueki S; Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Shido 1314-1, Samuki, Kagawa 769-2193, Japan.
Maruta S; Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.
Inoue A; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Arata T; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan. Electronic address: arata@bio.sci.osaka-u.ac.jp.

Biochem Biophys Res Commun ; 443(3): 911-6, 2014 Jan 17.

Article en En | MEDLINE | ID: mdl-24361895

Asunto(s)

Cinesinas/química; Cinesinas/metabolismo; Nucleótidos/metabolismo; Marcadores de Spin; Adenosina Trifosfatasas/metabolismo; Cristalografía por Rayos X; Espectroscopía de Resonancia por Spin del Electrón; Microtúbulos/metabolismo; Mutagénesis Sitio-Dirigida; Proteínas Mutantes/química; Estructura Secundaria de Proteína; Rotación

Palabras clave

2-mercaptoethanol; 4-maleimido-2,2,6,6-tetramethyl-1-piperidinoxy; AMPPNP; CW; Conventional kinesin; Dipolar EPR; EGTA; EM; Electron paramagnetic resonance; Kinesin-1; MSL; MT; Molecular motor; NN; Neck-linker; PIPES; SDSL; Spin labeling; Spinspin distance; Structural dynamics; adenosine 5'-(ß,Î³-imido)triphosphate; continuous wave; electron microscopy; ethylene glycol-bis(l-aminoethyl ether)-N,N,N',N'-tetraacetic acid; microtubule; no nucleotide; piperazine-1,4-bis(2-ethanesulfonic acid); site-directed spin labeling; ßME

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Marcadores de Spin / Cinesinas / Nucleótidos Tipo de estudio: Prognostic_studies Idioma: En Revista: Biochem Biophys Res Commun Año: 2014 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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