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In vivo silencing of the transcription factor IRF5 reprograms the macrophage phenotype and improves infarct healing.
Courties, Gabriel; Heidt, Timo; Sebas, Matthew; Iwamoto, Yoshiko; Jeon, Derrick; Truelove, Jessica; Tricot, Benoit; Wojtkiewicz, Greg; Dutta, Partha; Sager, Hendrik B; Borodovsky, Anna; Novobrantseva, Tatiana; Klebanov, Boris; Fitzgerald, Kevin; Anderson, Daniel G; Libby, Peter; Swirski, Filip K; Weissleder, Ralph; Nahrendorf, Matthias.
Afiliación
  • Courties G; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Heidt T; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Sebas M; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Iwamoto Y; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Jeon D; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Truelove J; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Tricot B; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Wojtkiewicz G; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Dutta P; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Sager HB; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Borodovsky A; Alnylam Pharmaceuticals, Cambridge, Massachusetts.
  • Novobrantseva T; Alnylam Pharmaceuticals, Cambridge, Massachusetts.
  • Klebanov B; Alnylam Pharmaceuticals, Cambridge, Massachusetts.
  • Fitzgerald K; Alnylam Pharmaceuticals, Cambridge, Massachusetts.
  • Anderson DG; David H. Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Division of Health Science Technology, and Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts.
  • Libby P; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
  • Swirski FK; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • Weissleder R; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts.
  • Nahrendorf M; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts. Electronic address: mnahrendorf@mgh.harvard.edu.
J Am Coll Cardiol ; 63(15): 1556-66, 2014 Apr 22.
Article en En | MEDLINE | ID: mdl-24361318
OBJECTIVES: The aim of this study was to test whether silencing of the transcription factor interferon regulatory factor 5 (IRF5) in cardiac macrophages improves infarct healing and attenuates post-myocardial infarction (MI) remodeling. BACKGROUND: In healing wounds, the M1 toward M2 macrophage phenotype transition supports resolution of inflammation and tissue repair. Persistence of inflammatory M1 macrophages may derail healing and compromise organ functions. The transcription factor IRF5 up-regulates genes associated with M1 macrophages. METHODS: Here we used nanoparticle-delivered small interfering ribonucleic acid (siRNA) to silence IRF5 in macrophages residing in MIs and in surgically-induced skin wounds in mice. RESULTS: Infarct macrophages expressed high levels of IRF5 during the early inflammatory wound-healing stages (day 4 after coronary ligation), whereas expression of the transcription factor decreased during the resolution of inflammation (day 8). Following in vitro screening, we identified an siRNA sequence that, when delivered by nanoparticles to wound macrophages, efficiently suppressed expression of IRF5 in vivo. Reduction of IRF5 expression, a factor that regulates macrophage polarization, reduced expression of inflammatory M1 macrophage markers, supported resolution of inflammation, accelerated cutaneous and infarct healing, and attenuated development of post-MI heart failure after coronary ligation as measured by protease targeted fluorescence molecular tomography-computed tomography imaging and cardiac magnetic resonance imaging (p < 0.05). CONCLUSIONS: This work identified a new therapeutic avenue to augment resolution of inflammation in healing infarcts by macrophage phenotype manipulation. This therapeutic concept may be used to attenuate post-MI remodeling and heart failure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Regulación de la Expresión Génica / Remodelación Ventricular / Factores Reguladores del Interferón / Macrófagos / Infarto del Miocardio / Miocardio Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Am Coll Cardiol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Regulación de la Expresión Génica / Remodelación Ventricular / Factores Reguladores del Interferón / Macrófagos / Infarto del Miocardio / Miocardio Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Am Coll Cardiol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos