Therapeutic potential of anti-IL-1ß IgY in guinea pigs with allergic asthma induced by ovalbumin.
Mol Immunol
; 58(1): 139-49, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24355520
BACKGROUND: Interleukin-1 beta (IL-1ß) plays pivotal roles in the progression of allergic airway inflammation. This study aims to determine whether the blockade of IL-1ß can inhibit airway inflammation in guinea pigs with allergic asthma induced by the inhalation of aerosolized ovalbumin (OVA). METHODS: Healthy guinea pigs treated with saline were used as normal controls (group C). The guinea pigs with allergic asthma induced by the inhalation of aerosolized OVA were randomly divided into three groups: (1) the M group containing negative control animals treated with saline; (2) the Z1 group containing animals treated by the inhalation of atomized 0.1% anti-IL-1ß immunoglobulin yolk (IgY); and (3) the Z2 group containing positive control animals that were treated with budesonide. The inflammatory cells in the peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were evaluated using methylene blue and eosin staining. Cytokine concentrations were measured using an enzyme-linked immunosorbent assay. Pulmonary sections were examined using hematoxylin-eosin staining. RESULTS: Allergic inflammation and damage to the pulmonary tissues were decreased in the Z1 group compared to the M group. Eosinophils and neutrophils in the PB and BALF were significantly decreased in the Z1 group compared to the M group (P<0.05). Treatment with anti-IL-1ß IgY significantly reduced the levels of IL-1ß, IL-4, IL-8, IL-13, TNF-α, TGF-ß1 and IgE in the BALF (P<0.05). CONCLUSION: The inhalation of aerosolized anti-IL-1ß IgY inhibits pathological responses in the pulmonary tissues of guinea pigs with allergic asthma. The inhibitory activity may be due to the decrease in the numbers of eosinophils and neutrophils and the reduced levels of inflammatory cytokines and IgE in the PB and BALF.
Palabras clave
AHR; Allergic asthma; BALF; Cytokines; Guinea pigs; HE; IL-1 receptor antagonist; IL-13; IL-1ra; IL-1ß; IL-4; IL-8; IgE; IgY; Immunoglobulin yolk; Infliximab; Interleukin-1ß; OVA; Omalizumab; PB; T helper type 2; TGF-ß(1); TNF-α; Th2; airway hyperresponsiveness; anti-IgE antibody; anti-TNF-α antibody; bronchoalveolar lavage fluid; hematoxylin-eosin; immunoglobulin E; immunoglobulin yolk; interleukin-13; interleukin-1beta; interleukin-4; interleukin-8; ovalbumin; peripheral blood; sRaw; sTNFR; soluble TNF-α receptor; specific airway resistance; transforming growth factor-beta 1; tumor necrosis factor-alpha
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Asma
/
Inmunoglobulinas
/
Interleucina-1beta
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Immunol
Año:
2014
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Reino Unido