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CUDC-101, a histone deacetylase inhibitor, improves the in vitro and in vivo developmental competence of somatic cell nuclear transfer pig embryos.
Jin, Jun-Xue; Li, Suo; Hong, Yu; Jin, Long; Zhu, Hai-Ying; Guo, Qing; Gao, Qing-Shan; Yan, Chang-Guo; Kang, Jin-Dan; Yin, Xi-Jun.
Afiliación
  • Jin JX; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Li S; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Hong Y; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Jin L; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Zhu HY; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Guo Q; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Gao QS; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Yan CG; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
  • Kang JD; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China. Electronic address: kangjindan@hotmail.com.
  • Yin XJ; Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China. Electronic address: yinxj33@msn.com.
Theriogenology ; 81(4): 572-8, 2014 Mar 01.
Article en En | MEDLINE | ID: mdl-24342668
The aim of the present study was to examine the effects of CUDC-101, a novel histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 at lysine 9 (AcH3K9) in pig SCNT embryos. We found that treatment with 1 µmol/L CUDC-101 for 24 hours significantly improved the development of pig SCNT embryos. Compared with the control group, the blastocyst rate was higher (18.5% vs. 10.3%; P < 0.05). To assess in vivo developmental potency, CUDC-101-treated SCNT embryos were transferred into two surrogate mothers, resulting in one pregnancy with six fetuses. We then investigated the acetylation level of histone H3K9 in SCNT embryos treated with CUDC-101 and compared them only against untreated embryos. The acetylation level of control SCNT embryos was lower than that of CUDC-101-treated embryos at pseudo-pronuclear stages, and immunofluorescent signal for H3K9ac in CUDC-101-treated embryos in a pattern similar to that of control group. In conclusion, we demonstrated that CUDC-101 can significantly improve in vitro and in vivo developmental competence and enhance the nuclear reprogramming of pig SCNT embryos.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quinazolinas / Porcinos / Histonas / Desarrollo Embrionario / Embrión de Mamíferos / Inhibidores Enzimáticos / Ácidos Hidroxámicos Límite: Animals / Pregnancy Idioma: En Revista: Theriogenology Año: 2014 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quinazolinas / Porcinos / Histonas / Desarrollo Embrionario / Embrión de Mamíferos / Inhibidores Enzimáticos / Ácidos Hidroxámicos Límite: Animals / Pregnancy Idioma: En Revista: Theriogenology Año: 2014 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos