Stabilization of translationally active mRNA by prokaryotic REP sequences.

Newbury, S F; Smith, N H; Robinson, E C; Hiles, I D; Higgins, C F

Newbury, S F; Smith, N H; Robinson, E C; Hiles, I D; Higgins, C F.

Cell ; 48(2): 297-310, 1987 Jan 30.

Article en En | MEDLINE | ID: mdl-2433046

RESUMEN

The REP sequence is a highly conserved inverted repeat that is present in about 25% of all E. coli transcription units. We show that the REP sequence can stabilize upstream RNA, independently of any other sequences, by protection from 3'-5' exonuclease attack. The REP sequence is frequently responsible for the differential stability of different segments of mRNA within an operon. We demonstrate that REP-stabilized mRNA can be translated in vivo and that cloning the REP sequence downstream of a gene can increase protein synthesis. This provides direct evidence that alterations in mRNA stability can play a role in determining bacterial gene expression. The implications of these findings for the mechanisms of mRNA degradation and for the role of RNA stability in the regulation of gene expression are discussed.

Asunto(s)

Regulación de la Expresión Génica; Biosíntesis de Proteínas; ARN Bacteriano/genética; ARN Mensajero/genética; Secuencias Repetitivas de Ácidos Nucleicos; Proteínas Bacterianas/biosíntesis; Proteínas Bacterianas/genética; ADN Bacteriano/genética; Escherichia coli/genética; Escherichia coli/metabolismo; Maltosa/metabolismo; Operón; ARN Bacteriano/metabolismo; ARN Mensajero/metabolismo; Salmonella typhimurium/genética; Salmonella typhimurium/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biosíntesis de Proteínas / ARN Bacteriano / ARN Mensajero / Secuencias Repetitivas de Ácidos Nucleicos / Regulación de la Expresión Génica Idioma: En Revista: Cell Año: 1987 Tipo del documento: Article Pais de publicación: Estados Unidos

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