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An integrated microfludic device for culturing and screening of Giardia lamblia.
Zheng, Guo-Xia; Zhang, Xue-Mei; Yang, Yu-Suo; Zeng, Shu-Rui; Wei, Jun-Feng; Wang, Yun-Hua; Li, Ya-Jie.
Afiliación
  • Zheng GX; Environmental and Chemical Engineering School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China.
  • Zhang XM; Medical School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China.
  • Yang YS; Environmental and Chemical Engineering School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China.
  • Zeng SR; Medical School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China.
  • Wei JF; Environmental and Chemical Engineering School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China.
  • Wang YH; Medical School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China. Electronic address: yunhua1977@126.com.
  • Li YJ; Medical School, Dalian University, Dalian 116622, PR China; Microfluidic Research Institute, Dalian University, Dalian 116622, PR China. Electronic address: liyj@dlu.edu.cn.
Exp Parasitol ; 137: 1-7, 2014 Feb.
Article en En | MEDLINE | ID: mdl-24316463
In vitro culturing of trophozoites was important for research of Giardia lamblia (G. lamblia), especially in discovery of anti-Giardia agents. The current culture methods mainly suffer from lab-intension or the obstacle in standardizing the gas condition. Thus, it could benefit from a more streamlined and integrated approach. Microfluidics offers a way to accomplish this goal. Here we presented an integrated microfluidic device for culturing and screening of G. lamblia. The device consisted of a polydimethylsiloxane (PDMS) microchip with an aerobic culture system. In the microchip, the functionality of integrated concentration gradient generator (CGG) with micro-scale cell culture enables dose-response experiment to be performed in a simple and reagent-saving way. The diffusion-based culture chambers allowed growing G. lamblia at the in vivo like environment. It notable that the highly air permeable material of parallel chambers maintain uniform anaerobic environment in different chambers easily. Using this device, G. lamblia were successfully cultured and stressed on-chip. In all cases, a dose-related inhibitory response was detected. The application of this device for these purposes represents the first step in developing a completely integrated microfluidic platform for high-throughput screening and might be expanded to other assays based on in vitro culture of G. lamblia with further tests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Giardia lamblia / Microfluídica / Técnicas Analíticas Microfluídicas / Antiprotozoarios Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Exp Parasitol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Giardia lamblia / Microfluídica / Técnicas Analíticas Microfluídicas / Antiprotozoarios Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Exp Parasitol Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos