Functional characterization of ArabidopsisâHsfA6a as a heat-shock transcription factor under high salinity and dehydration conditions.

Hwang, Sung Min; Kim, Dae Won; Woo, Min Seok; Jeong, Hyeong Seop; Son, Young Sim; Akhter, Salina; Choi, Gyung Ja; Bahk, Jeong Dong

Functional characterization of ArabidopsisâHsfA6a as a heat-shock transcription factor under high salinity and dehydration conditions.

Hwang, Sung Min; Kim, Dae Won; Woo, Min Seok; Jeong, Hyeong Seop; Son, Young Sim; Akhter, Salina; Choi, Gyung Ja; Bahk, Jeong Dong.

Afiliación

Hwang SM; Division of Applied Life Sciences (BK21+), Graduate School of Gyeongsang National University, Jinju, 660-701, Korea; Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Deajeon, 305-600, Korea.

Plant Cell Environ ; 37(5): 1202-22, 2014 May.

Article en En | MEDLINE | ID: mdl-24313737

RESUMEN

Although heat-shock transcription factors are well characterized in the heat stress-related pathway, they are poorly understood in other stress responses. Here, we functionally characterized AtHsfA6a in the presence of exogenous abscisic acid (ABA) and under high salinity and dehydration conditions. AtHsfA6a expression under normal conditions is very low, but was highly induced by exogenous ABA, NaCl and drought. Unexpectedly, the levels of AtHsfA6a transcript were not significantly altered under heat and cold stresses. Electrophoretic mobility shift assays and transient transactivation assays indicated that AtHsfA6a is transcriptionally regulated by ABA-responsive element binding factor/ABA-responsive element binding protein, which are key regulators of the ABA signalling pathway. Additionally, fractionation and protoplast transient assays showed that AtHsfA6a was in cytoplasm and nucleus simultaneously; however, under conditions of high salinity the majority of AtHsfA6A was in the nucleus. Furthermore, at both seed germination and seedlings stage, plants overexpressing AtHsfA6a were hypersensitive to ABA and exhibited enhanced tolerance against salt and drought stresses. Finally, the microarray and qRT-PCR analyses revealed that many stress-responsive genes were up-regulated in the plants overexpressing AtHsfA6a. Taken together, the data strongly suggest that AtHsfA6a acts as a transcriptional activator of stress-responsive genes via the ABA-dependent signalling pathway.

Asunto(s)

Proteínas de Arabidopsis/metabolismo; Arabidopsis/fisiología; Proteínas de Unión al ADN/metabolismo; Salinidad; Factores de Transcripción/metabolismo; Ácido Abscísico/farmacología; Adaptación Fisiológica/efectos de los fármacos; Adaptación Fisiológica/genética; Arabidopsis/efectos de los fármacos; Arabidopsis/genética; Arabidopsis/crecimiento & desarrollo; Proteínas de Arabidopsis/química; Proteínas de Arabidopsis/genética; Núcleo Celular/efectos de los fármacos; Núcleo Celular/metabolismo; ADN Bacteriano/genética; Proteínas de Unión al ADN/química; Proteínas de Unión al ADN/genética; Deshidratación; Sequías; Regulación de la Expresión Génica de las Plantas/efectos de los fármacos; Factores de Transcripción del Choque Térmico; Mutagénesis Insercional/genética; Regiones Promotoras Genéticas/genética; Unión Proteica/efectos de los fármacos; Unión Proteica/genética; Reproducibilidad de los Resultados; Saccharomyces cerevisiae/citología; Transducción de Señal/efectos de los fármacos; Transducción de Señal/genética; Fracciones Subcelulares/efectos de los fármacos; Fracciones Subcelulares/metabolismo; Factores de Transcripción/química; Factores de Transcripción/genética; Activación Transcripcional/efectos de los fármacos; Activación Transcripcional/genética; Regulación hacia Arriba/efectos de los fármacos; Regulación hacia Arriba/genética

Palabras clave

ABA-dependent signalling pathway; ABF/AREB proteins; AtHsfA6a; abscisic acid (ABA); drought; transcriptional activator

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Arabidopsis / Proteínas de Arabidopsis / Proteínas de Unión al ADN / Salinidad Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2014 Tipo del documento: Article Pais de publicación: Estados Unidos

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