A Pasteurella multocida sialyltransferase displaying dual trans-sialidase activities for production of 3'-sialyl and 6'-sialyl glycans.
J Biotechnol
; 170: 60-7, 2014 Jan 20.
Article
en En
| MEDLINE
| ID: mdl-24291191
Palabras clave
2-O-(p-nitrophenyl)-α-d-N-acetylneuraminic acid; 4-methylumbelliferyl ß-d-lactoside; ATP; CMP-NeuAc; CTP; CV; Casein glycomacropeptide as source for sialic acid; Enzyme expression and purification; GOS; GalNAc; HMOs; HPAEC-PAD; Human milk oligosaccharides; Kinetics of trans-sialidase; LCMS; LacMu; MALDI-MS; N-His6-tag; N-acetylgalactosamine; N-acetylneuraminic acid; N-terminal His(6)-tag; NDV; Neu5Ac; Newcastle disease virus; PEP; PGC; Pasteurella multocida sialyltransferase; PmST; RSM; Response surface; SDS-PAGE; Sia-GOS; TLC; adenosine-5'-triphosphate; cGMP; capillary liquid chromatography/mass spectrometry; casein glycomacropeptide; column volume; cytidine-5'-monophospho-N-acetylneuraminic acid; cytidine-5'-triphosphate; galactooligosaccharides; high-performance anion exchange chromatography; human milk oligosaccharides; matrix-assisted laser desorption/ionization mass spectrometry; pNP-Neu5Ac; phosphoenolpyruvate; porous graphitic carbon; response surface methodology; sialylated galactooligosaccharides; sodium dodecyl sulfate polyacrylamide gel electrophoresis; thin-layer chromatography
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligosacáridos
/
Sialiltransferasas
/
Glicoproteínas
/
Pasteurella multocida
/
Ácido N-Acetilneuramínico
/
Lactosa
/
Proteínas de la Leche
/
Neuraminidasa
Límite:
Humans
Idioma:
En
Revista:
J Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Dinamarca
Pais de publicación:
Países Bajos