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Novel pandemic influenza A (H1N1) virus infection modulates apoptotic pathways that impact its replication in A549 cells.
Wang, Xue; Tan, Jiying; Zoueva, Olga; Zhao, Jiangqin; Ye, Zhiping; Hewlett, Indira.
Afiliación
  • Wang X; Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Bethesda, MD 20892, USA. Electronic address: xue.wang@fda.hhs.gov.
  • Tan J; Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Bethesda, MD 20892, USA.
  • Zoueva O; Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
  • Zhao J; Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Bethesda, MD 20892, USA.
  • Ye Z; Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
  • Hewlett I; Lab of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Bethesda, MD 20892, USA.
Microbes Infect ; 16(3): 178-86, 2014 Mar.
Article en En | MEDLINE | ID: mdl-24262752
It is not well-known whether apoptosis signaling affects influenza virus infection and reproduction in human lung epithelial cells. Using A549 cell line, we studied the relationship of some apoptosis-associated molecules with novel pandemic influenza A (H1N1) virus, A/California/04/2009. Infected cells displayed upregulated Fas ligand, activated FADD and caspase-8, and downregulated FLIP in the extrinsic apoptotic pathway. p53 expression increased and Bcl-XL expression decreased in the intrinsic pathway. Expression of pre-apoptotic molecules (FasL, FADD, and p53) increased virus replication, while inhibition of activity of FADD, caspase-8 and caspase-3, and expression of anti-apoptotic proteins (FLIP and Bcl-XL) decreased virus replication. p38, ERK and JNK from MAPK pathways were activated in infected cells, and inhibition with their inhibitors diminished virus replication. In the p38 superfamily, p38α expression increased viral RNA production, while expression of p38ß and p38γ decreased. These data indicated that influenza virus induces apoptotic signaling pathways, which benefit virus replication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Apoptosis / Gripe Humana / Subtipo H1N1 del Virus de la Influenza A Límite: Humans Idioma: En Revista: Microbes Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Apoptosis / Gripe Humana / Subtipo H1N1 del Virus de la Influenza A Límite: Humans Idioma: En Revista: Microbes Infect Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Año: 2014 Tipo del documento: Article Pais de publicación: Francia