AgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L.
Plant Cell Physiol
; 55(1): 229-36, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-24259681
The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). The procedure of the AgarTrap was carried out by simply exchanging appropriate solutions in a Petri dish, and completed within a week, successfully yielding sufficient numbers of independent transformants for molecular analysis (e.g. characterization of gene/protein function) in a single experiment. The AgarTrap method will promote future molecular biological study in M. polymorpha.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transformación Genética
/
Técnicas Genéticas
/
Marchantia
/
Agrobacterium
Idioma:
En
Revista:
Plant Cell Physiol
Asunto de la revista:
BOTANICA
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Japón