Somatic embryogenesis and plants from zygotic embryos of coconut (Cocos nucifera L.) in vitro.
Plant Cell Rep
; 3(6): 222-5, 1984 Dec.
Article
en En
| MEDLINE
| ID: mdl-24253571
Complete plants were grown from zygotic embryos cultured on Y3 basal liquid medium supplemented with coconut milk, BA and NAA. Explants from stem, leaf and rachilla of mature coconut trees turned green and swelled on Y3 semi-solid basal media supplemented with 2,4-D, K, NAA, BA and activated charcoal. Callus was initiated in explants from the subapical regions of the stem on Y3 basal medium supplemented with 2,4-D (4.52×10(2)µM). Globular embryo-like structures were obtained when this callus was subcultured to auxinless medium. Root formation was obtained from leaf explants on Y3 basal medium containing citric acid, ascorbic acid and 2,4-D (4.52×10(2) µM). Globular embryo-like structures were also obtained directly from leaf explants on a Y3 basal medium supplemented with 2,4-D (2.26×10(2) µM). Callus isolated from rachilla explants on Y3 basal medium containing 2,4-D(4.52×10(2) µM), formed nodular structures when transferred to medium with 2,4-D (2.3×10(1) µM). These nodules developed roots from the base of the nodular growth whereas from the upper portion shoots were observed on Y3 basal liquid medium.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Plant Cell Rep
Asunto de la revista:
BOTANICA
Año:
1984
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Alemania