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Quantitative real-time PCR for detection of neurotoxin genes of Clostridium botulinum types A, B and C in equine samples.
Johnson, Amy L; McAdams-Gallagher, Susan C; Sweeney, Raymond W.
Afiliación
  • Johnson AL; Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA 19348, USA. Electronic address: amyjohn@vet.upenn.edu.
  • McAdams-Gallagher SC; Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA 19348, USA.
  • Sweeney RW; Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA 19348, USA.
Vet J ; 199(1): 157-61, 2014 Jan.
Article en En | MEDLINE | ID: mdl-24252222
Botulism in horses in the USA is attributed to Clostridium botulinum types A, B or C. In this study, a duplex quantitative real-time PCR (qPCR) for detection of the neurotoxin genes of C. botulinum types A and B, and a singleplex qPCR for detection of the neurotoxin gene of C. botulinum type C, were optimized and validated for equine gastrointestinal, faecal and feed samples. The performance of these assays was evaluated and compared to the standard mouse bioassay (MBA) using 148 well-characterized samples, most of which were acquired from a repository of veterinary diagnostic samples from cases of botulism: 106 samples positive for C. botulinum (25 type A, 27 type B, 28 type C, 1 type D and 25 type E) and 42 negative samples. The sensitivities of the qPCR assays were 89%, 86% and 96% for C. botulinum types A, B and C, respectively. The overall sensitivity of the mouse bioassay for types A, B and C was 81%. The specificities of the qPCR assays were 99-100% and the specificity of the mouse bioassay was 95%.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clostridium botulinum tipo A / Clostridium botulinum tipo B / Clostridium botulinum tipo C / Reacción en Cadena en Tiempo Real de la Polimerasa / Caballos / Neurotoxinas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clostridium botulinum tipo A / Clostridium botulinum tipo B / Clostridium botulinum tipo C / Reacción en Cadena en Tiempo Real de la Polimerasa / Caballos / Neurotoxinas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2014 Tipo del documento: Article Pais de publicación: Reino Unido