Family-wide expression characterization of Arabidopsis beta-carbonic anhydrase genes using qRT-PCR and Promoter::GUS fusions.

Wang, Meng; Zhang, Qiong; Liu, Fang-Chun; Xie, Wei-Fa; Wang, Guang-Dong; Wang, Jun; Gao, Qing-Hua; Duan, Ke

Wang, Meng; Zhang, Qiong; Liu, Fang-Chun; Xie, Wei-Fa; Wang, Guang-Dong; Wang, Jun; Gao, Qing-Hua; Duan, Ke.

Afiliación

Wang M; Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China; Biotech Research Institute, SAAS, Shanghai 201106, China; College of Life Sciences, Ningxia University, Yinchuan 750021
Zhang Q; Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China; Biotech Research Institute, SAAS, Shanghai 201106, China; College of Horticultural Sciences, Nanjing Agricultural Unive
Liu FC; Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China.
Xie WF; Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China.
Wang GD; College of Horticultural Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Wang J; College of Life Sciences, Ningxia University, Yinchuan 750021, China.
Gao QH; Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China. Electronic address: qhgao20338@sina.com.
Duan K; Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, China; Biotech Research Institute, SAAS, Shanghai 201106, China. Electronic address: kduan936@126.com.

Biochimie ; 97: 219-27, 2014 Feb.

Article en En | MEDLINE | ID: mdl-24211190

RESUMEN

Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes found throughout the phylogenetic tree. The ß-class carbonic anhydrases (ß-CAs) are the predominating class of CAs in plants. Growing evidence underscores the importance of ß-CAs in plant immunity and environmental adaptation in addition to their roles in photosynthesis. However, many fundamental problems in Arabidopsis ßCAs expression remain unsolved. Here we examined the transcript abundance of AtßCAs in different tissues of Arabidopsis thaliana, and the accumulation of mRNA in response to CO2 and darkness. Histochemical analysis was performed to study the promoter activity of AtßCAs during post-germination seedling growth and in mature plants. All six members of the AtßCA subfamily showed a response to changed CO2 level and darkness, but each member showed a specific dynamic pattern. Although expression of each AtßCA was unique, in general most AtßCAs were synchronously expressed in green leaves since 5 days after germination until flowering. AtßCA1 and AtßCA2 were most highly expressed in leaves but AtßCA2 displayed weaker expression in roots. The level of AtßCA3 transcripts was highest in flowers, while AtßCA5 was most widely expressed and might be involved in more processes than other members. AtßCA6 was unique for increased expression in darkness and no expression in either the anther or pistil. The present study provides useful information for further functional investigation.

Asunto(s)

Proteínas de Arabidopsis/genética; Arabidopsis/genética; Anhidrasas Carbónicas/genética; Flores/genética; Regulación de la Expresión Génica de las Plantas; Hojas de la Planta/genética; Raíces de Plantas/genética; Arabidopsis/enzimología; Proteínas de Arabidopsis/metabolismo; Dióxido de Carbono/metabolismo; Anhidrasas Carbónicas/metabolismo; Oscuridad; Flores/enzimología; Isoenzimas/genética; Isoenzimas/metabolismo; Especificidad de Órganos; Hojas de la Planta/enzimología; Raíces de Plantas/enzimología; Regiones Promotoras Genéticas; ARN Mensajero/genética; ARN Mensajero/metabolismo

Palabras clave

Arabidopsis; Beta-carbonic anhydrase gene; CA; CO(2); CO(2) concentration; DAG; Environmental response; PEPC; Plant development; UTR; carbon dioxide; carbonic anhydrase; days after germination; phosphoenol pyruvate carboxylase; qRT-PCR; quantitative reverse transcription PCR; untranslated region

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anhidrasas Carbónicas / Arabidopsis / Raíces de Plantas / Hojas de la Planta / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Flores Idioma: En Revista: Biochimie Año: 2014 Tipo del documento: Article Pais de publicación: Francia

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