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Expression of 11ß-hydroxysteroid dehydrogenase type 2 is deregulated in colon carcinoma.
Moravec, Martin; Svec, Jirí; Ergang, Peter; Mandys, Václav; Reháková, Lenka; Zádorová, Zdena; Hajer, Jan; Kment, Milan; Pácha, Jirí.
Afiliación
  • Moravec M; Institute of Physiology, Academy of Sciences of the Czech Republic, and Second Department of Internal Medicine, Third Faculty of Medicine, Prague, Czech Republic.
  • Svec J; Institute of Physiology, Academy of Sciences of the Czech Republic, and Second Department of Internal Medicine, Third Faculty of Medicine, Prague, Czech Republic.
  • Ergang P; Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
  • Mandys V; Department of Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Reháková L; Department of Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Zádorová Z; Second Department of Internal Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Hajer J; Second Department of Internal Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Kment M; Second Department of Internal Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Pácha J; Institute of Physiology, Academy of Sciences of the Czech Republic, and Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic. pacha@biomed.cas.cz.
Histol Histopathol ; 29(4): 489-96, 2014 Apr.
Article en En | MEDLINE | ID: mdl-24189979
Although the effects of glucocorticoids on proliferation, differentiation and apoptosis are well known, and steroid hormones have been identified to play a role in pathogenesis and the development of various cancers, limited data are available regarding the relationship between the local metabolism of glucocorticoids and colorectal adenocarcinoma (CRC) formation. Glucocorticoid metabolism is determined by 11ß-hydroxysteroid dehydrogenases type 1 and 2 (11HSD1, 11HSD2), which increase the local concentration of cortisol due to the reduction of cortisone, or decrease this concentration due to the oxidation of cortisol. The objective of this study was to evaluate the extent of 11HSD1 and 11HSD2 mRNA in pre-malignant colorectal polyps and in CRC. The specimens were retrieved from patients by endoscopic or surgical resection and the expression of 11HSD1 and 11HSD2 was measured by real-time PCR. The polyps were of the following histological types: hyperplastic polyps and adenomas with low- or high-grade dysplasia. The neoplastic tissue of CRC obtained during tumor surgery was also studied. It was found that 11HSD2 was not only downregulated in CRC but already in the early stages of neoplastic transformation (adenoma with low-grade dysplasia). In contrast, the level of 11HSD1 was significantly increased in CRC but not in pre-malignant polyps. The results demonstrate that the downregulation of 11HSD2 gene expression is a typical feature of the development of colorectal polypous lesions and their transformation into CRC.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Adenocarcinoma / Biomarcadores de Tumor / 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2 Límite: Adolescent / Adult / Aged / Aged80 / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Histol Histopathol Asunto de la revista: HISTOLOGIA / PATOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: España

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Adenocarcinoma / Biomarcadores de Tumor / 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2 Límite: Adolescent / Adult / Aged / Aged80 / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Histol Histopathol Asunto de la revista: HISTOLOGIA / PATOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: España