Neonatal tissue injury reduces the intrinsic excitability of adult mouse superficial dorsal horn neurons.
Neuroscience
; 256: 392-402, 2014 Jan 03.
Article
en En
| MEDLINE
| ID: mdl-24184978
Tissue damage during the neonatal period evokes long-lasting changes in nociceptive processing within the adult spinal cord which contribute to persistent alterations in pain sensitivity. However, it remains unclear if the observed modifications in neuronal activity within the mature superficial dorsal horn (SDH) following early injury reflect shifts in the intrinsic membrane properties of these cells. Therefore, the present study was undertaken to identify the effects of neonatal surgical injury on the intrinsic excitability of both GABAergic and presumed glutamatergic neurons within lamina II of the adult SDH using in vitro patch clamp recordings from spinal cord slices prepared from glutamic acid decarboxylase-green fluorescent protein (Gad-GFP) mice. The results demonstrate that hindpaw surgical incision at postnatal day (P) 3 altered the passive membrane properties of both Gad-GFP and adjacent, non-GFP neurons in the mature SDH, as evidenced by decreased membrane resistance and more negative resting potentials in comparison to naïve littermate controls. This was accompanied by a reduction in the prevalence of spontaneous activity within the GABAergic population. Both Gad-GFP and non-GFP neurons displayed a significant elevation in rheobase and decreased instantaneous firing frequency after incision, suggesting that early tissue damage lowers the intrinsic membrane excitability of adult SDH neurons. Isolation of inward-rectifying K(+) (K(ir)) currents revealed that neonatal incision significantly increased K(ir) conductance near physiological membrane potentials in GABAergic, but not glutamatergic, lamina II neurons. Overall, these findings suggest that neonatal tissue injury causes a long-term dampening of intrinsic firing across the general population of lamina II interneurons, but the underlying ionic mechanisms may be cell-type specific.
Palabras clave
ANOVA; AP; C(m); E(rev); Gad-GFP; HEPES; IF; K(ir); N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid; R(m); SDH; V(m); V(rest); aCSF; action potential; analysis of variance; artificial cerebrospinal fluid; development; eGFP; enhanced GFP; glutamic acid decarboxylase-green fluorescent protein; incision; instantaneous firing frequency; inward-rectifying K(+); lamina II; membrane capacitance; membrane potential; membrane resistance; patch clamp; resting membrane potential; reversal potential; spinal cord; superficial dorsal horn
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Potenciales de Acción
/
Ganglios Espinales
/
Neuronas
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
2014
Tipo del documento:
Article
Pais de publicación:
Estados Unidos