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Neutralization of cobra venom by cocktail antiserum against venom proteins of cobra (Naja naja naja).
Venkatesan, C; Sarathi, M; Balasubramanaiyan, G; Vimal, S; Madan, N; Sundar Raj, N; Mohammed Yusuf Bilal, S; Nazeer Basha, A; Farook, M A; Sahul Hameed, A S; Sridevi, G.
Afiliación
  • Venkatesan C; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India.
  • Sarathi M; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India; Department of Biology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada.
  • Balasubramanaiyan G; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India; Department of Zoology, Arignar Anna Govt Arts College, Cheiyar, Tamil Nadu, India.
  • Vimal S; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India.
  • Madan N; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India.
  • Sundar Raj N; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India.
  • Mohammed Yusuf Bilal S; Raja Muthaih Medical College, Annamalai University, Chidambaram, India.
  • Nazeer Basha A; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India.
  • Farook MA; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India.
  • Sahul Hameed AS; Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore Dist., Tamil Nadu, India. Electronic address: cah_sahul@hotmail.com.
  • Sridevi G; King Institute of Preventive Medicine, Chennai, Tamil Nadu, India.
Biologicals ; 42(1): 8-21, 2014 Jan.
Article en En | MEDLINE | ID: mdl-24176716
Naja naja venom was characterized by its immunochemical properties and electrophoretic pattern which revealed eight protein bands (14 kDa, 24 kDa, 29 kDa, 45 kDa, 48 kDa, 65 kDa, 72 kDa and 99 kDa) by SDS-PAGE in reducing condition after staining with Coomassie Brilliant Blue. The results showed that Naja venom presented high lethal activity. Whole venom antiserum or individual venom protein antiserum (14 kDa, 29 kDa, 65 kDa, 72 kDa and 99 kDa) of venom could recognize N. naja venom by Western blotting and ELISA, and N. naja venom presented antibody titer when assayed by ELISA. The neutralization tests showed that the polyvalent antiserum neutralized lethal activities by both in vivo and in vitro studies using mice and Vero cells. The antiserum could neutralize the lethal activities in in-vivo and antivenom administered after injection of cobra venom through intraperitoneal route in mice. The cocktail antiserum also could neutralize the cytotoxic activities in Vero cell line by MTT and Neutral red assays. The results of the present study suggest that cocktail antiserum neutralizes the lethal activities in both in vitro and in vivo models using the antiserum against cobra venom and its individual venom proteins serum produced in rabbits.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Neutralización / Venenos Elapídicos / Sueros Inmunes Límite: Animals Idioma: En Revista: Biologicals Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Neutralización / Venenos Elapídicos / Sueros Inmunes Límite: Animals Idioma: En Revista: Biologicals Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido