Linkage Arrangement of RFLP loci in progenies from crosses between doubled haploid Asparagus officinalis L. clones.
Theor Appl Genet
; 90(1): 124-8, 1995 Jan.
Article
en En
| MEDLINE
| ID: mdl-24173793
A preliminary genetic map of the dioecious species Asparagus officinalis L. (2n = 20) has been constructed on the basis of restriction fragment length polymorphism (RFLP) and isozyme marker data. With DNA samples digested with either EcoRI or HindIII 61 out of 148 probes (41%) identified RFLPs in six families of doubled haploid lines obtained through anther culture. A higher level of polymorphism (65%) was observed when a single family was screened for RFLPs using six distinct restriction enzymes. Segregation analysis of the BC progenies (40-80 individuals) resulted in a 418-cM extended map comprising 43 markers: 39 RFLPs, three isozymes and one morphological (sex). These markers are clustered in 12 linkage groups and four of them exhibited significant deviations from the expected 1â¶1 ratio. One isozyme and three RFLP markers were assigned to the sex chromosome.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Theor Appl Genet
Año:
1995
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Alemania