Biological response modifiers released by a mezerein-treated human monocytic leukemia cell line, THP-1.
J Biol Response Mod
; 4(4): 396-407, 1985 Aug.
Article
en En
| MEDLINE
| ID: mdl-2411879
After mezerein treatment of suspension cultures of the acute monocytic human leukemia cell line THP-1, cells became adherent to plastic culture surfaces, lost division potential, acquired Fc receptors, displayed phagocytic activity, and expressed increased nonspecific esterase staining. Serum-free RPMI 1640 medium conditioned by adherent THP-1 cells was examined for the presence of biological response modifiers. Preparative isoelectrofocusing of concentrated medium in the presence of various pH gradients of Ampholine ampholytes resulted in the separation of the following activities: fibroblast growth-stimulating activities in pH ranges of 4.10-4.55 and 5.30-5.45; colony-stimulating factor (CSF) for mouse bone marrow cells at pH 4.10-4.55; and a malignant cell growth inhibitor comigrating with a lymphocyte-activating factor (interleukin-1) at pH 6.70-6.95. Both CSF and the fibroblast growth stimulator isofocused at pH 4.10-4.55 coeluted following molecular-sieve chromatography through P-100. CSF-induced colonies were composed of nongranulocytic mononuclear cells. Chromatography through an ACA-54 column separated interleukin-1 from most of the growth-inhibitory activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Terpenos
/
Leucemia Mieloide
/
Sustancias de Crecimiento
/
Diterpenos
/
Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
En
Revista:
J Biol Response Mod
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos