Location and possible function of fibronectin and laminin in clones of chick retinal pigmented epithelial cells.
In Vitro Cell Dev Biol
; 21(2): 79-87, 1985 Feb.
Article
en En
| MEDLINE
| ID: mdl-2409071
Distribution and organization of the extracellular glycoproteins, fibronectin and laminin, in clonal cultures of chick retinal pigmented epithelial cells have been investigated using indirect immunofluorescence microscopy. Fibronectin is located on the apical and basal cell surfaces and between the cells in the undifferentiated regions of the colony (outer edge and stratified region). It seems to run parallel to intracellular microfilament bundles and to be associated with them across the cell membrane. In the differentiated region of the colony (center), it is located exclusively on the basal cell surface and seems to be primarily associated with the collagen bundles of the basement membrane. The locations suggest that it may be necessary to permit rapid division and movement of the undifferentiated cells in the outer region of the colony, while stabilizing the sheet of differentiated cells in the colony center. In all regions except the outer edge of the colony, laminin is associated with the basal cell surfaces where it forms a meshwork of short, fine strands. The laminin has a totally different staining pattern from the fibronectin and does not seem to be associated with collagen bundles. The location suggests that laminin may be present in the basal lamina and may be involved in adhesion of the cells to the substratum.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Epitelio Pigmentado Ocular
/
Fibronectinas
/
Laminina
Límite:
Animals
Idioma:
En
Revista:
In Vitro Cell Dev Biol
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos