Hapten-specific T-cell response to azobenzenearsonate-N-acetyl-L-tyrosine in the Lewis rat. IV. Rat dendritic cells present soluble and insoluble azobenzenearsonate conjugates to T cells.
Cell Immunol
; 93(2): 447-58, 1985 Jul.
Article
en En
| MEDLINE
| ID: mdl-2408768
We have used a fractionation procedure involving bovine serum albumin gradient floatation, adherence to glass, and rosetting with antibody-coated sheep erythrocytes to purify an accessory cell fraction from Lewis rat spleens. The fraction which is of light buoyant density, nonadherent, and FcR- is markedly Ia+, lacks phagocytic ability, is nonspecific esterase negative and under scanning electron microscopy has a heterogeneous morphology with a variety of protuberences described for rat dendritic cells. This putative dendritic cell preparation is very effective at stimulating proliferative responses with concanavalin A and allogeneic cells. When used to reconstitute the reactivity of peritoneal exudate cells of rats immunized with azobenzenearsonate-N-acetyl-L-tyrosine (ABA-Tyr) and subsequently depleted of Ia+ cells, it was shown to be highly effective with ABA conjugates of tyrosine, Ficoll, and polystyrene beads. Thus, despite the apparent absence of phagocytic or degradative abilities, this cell was very efficient at presenting large soluble and insoluble antigens. It is suggested that processing may occur at the cell surface without requiring internalization.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
P-Azobencenoarsonato
/
Compuestos Azo
/
Tirosina
/
Linfocitos T
/
Haptenos
Límite:
Animals
Idioma:
En
Revista:
Cell Immunol
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Países Bajos