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The polyketide synthase gene pks4 of Trichoderma reesei provides pigmentation and stress resistance.
Atanasova, Lea; Knox, Benjamin P; Kubicek, Christian P; Druzhinina, Irina S; Baker, Scott E.
Afiliación
  • Atanasova L; Microbiology Group, Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
Eukaryot Cell ; 12(11): 1499-508, 2013 Nov.
Article en En | MEDLINE | ID: mdl-24036343
Species of the fungal genus Trichoderma (Hypocreales, Ascomycota) are well-known for their production of various secondary metabolites. Nonribosomal peptides and polyketides represent a major portion of these products. In a recent phylogenomic investigation of Trichoderma polyketide synthase (PKS)-encoding genes, the pks4 from T. reesei was shown to be an orthologue of pigment-forming PKSs involved in synthesis of aurofusarin and bikaverin in Fusarium spp. In this study, we show that deletion of this gene in T. reesei results in loss of green conidial pigmentation and in pigmentation alteration of teleomorph structures. It also has an impact on conidial cell wall stability and the antagonistic abilities of T. reesei against other fungi, including formation of inhibitory metabolites. In addition, deletion of pks4 significantly influences the expression of other PKS-encoding genes of T. reesei. To our knowledge, this is the first indication that a low-molecular-weight pigment-forming PKS is involved in defense, mechanical stability, and stress resistance in fungi.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Trichoderma / Pigmentación / Proteínas Fúngicas / Sintasas Poliquetidas Idioma: En Revista: Eukaryot Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2013 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Trichoderma / Pigmentación / Proteínas Fúngicas / Sintasas Poliquetidas Idioma: En Revista: Eukaryot Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2013 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos