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Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine.
Besse-Patin, A; Montastier, E; Vinel, C; Castan-Laurell, I; Louche, K; Dray, C; Daviaud, D; Mir, L; Marques, M-A; Thalamas, C; Valet, P; Langin, D; Moro, C; Viguerie, N.
Afiliación
  • Besse-Patin A; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
  • Montastier E; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [3] Nutrition and Clinical Biochemistry Departments, Toulouse University Hospitals, Toulouse, Fr
  • Vinel C; 1] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [2] Inserm, UMR1048, Adipolab, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.
  • Castan-Laurell I; 1] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [2] Inserm, UMR1048, Adipolab, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.
  • Louche K; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
  • Dray C; 1] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [2] Inserm, UMR1048, Adipolab, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.
  • Daviaud D; 1] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [2] Inserm, UMR1048, Adipolab, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.
  • Mir L; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
  • Marques MA; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
  • Thalamas C; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [3] Clinical Investigation Centre Inserm, CIC-9302, Department of Clinical Pharmacology, Toulous
  • Valet P; 1] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [2] Nutrition and Clinical Biochemistry Departments, Toulouse University Hospitals, Toulouse, France.
  • Langin D; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France [3] Nutrition and Clinical Biochemistry Departments, Toulouse University Hospitals, Toulouse, Fr
  • Moro C; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
  • Viguerie N; 1] Inserm, UMR1048, Obesity Research Laboratory, I2MC, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France [2] University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
Int J Obes (Lond) ; 38(5): 707-13, 2014 May.
Article en En | MEDLINE | ID: mdl-23979219
BACKGROUND: It has been suggested that the metabolic benefits of physical exercise could be mediated by myokines. We examined here the effect of exercise training on skeletal muscle expression of a panel of myokines in humans. Pathways regulating myokine expression were investigated in human myotubes. METHODS: Eleven obese non-diabetic male subjects were enrolled in an 8-week endurance training program. Insulin sensitivity was assessed by an oral glucose tolerance test. Subcutaneous adipose tissue and Vastus lateralis muscle biopsy samples were collected before and after training. RNAs were prepared from adipose tissue and skeletal muscle. Primary culture of myoblasts was established. RESULTS: As expected, exercise training improved aerobic capacity and decreased fat mass. No significant change in interleukin 6, fibroblast growth factor 21, myostatin (MSTN) or irisin mRNA level was found in muscle after training. A twofold increase in apelin mRNA level was found in muscle but not in adipose tissue. No change in circulating myokine and adipokine plasma levels was observed in the resting state in response to training. Interestingly, apelin was significantly expressed and secreted in primary human myotubes. Apelin gene expression was upregulated by cyclic AMP and calcium, unlike the other myokines investigated. Importantly, changes in muscle apelin mRNA levels were positively related to whole-body insulin sensitivity improvement. CONCLUSION: Collectively, our data show that exercise training upregulates muscle apelin expression in obese subjects. Apelin expression is induced by exercise signaling pathways and secreted in vitro in human primary myotubes, and may behave as a novel exercise-regulated myokine with autocrine/paracrine action.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia Física / Ejercicio Físico / Músculo Esquelético / Fibras Musculares Esqueléticas / Obesidad Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Humans / Male Idioma: En Revista: Int J Obes (Lond) Asunto de la revista: METABOLISMO Año: 2014 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia Física / Ejercicio Físico / Músculo Esquelético / Fibras Musculares Esqueléticas / Obesidad Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Humans / Male Idioma: En Revista: Int J Obes (Lond) Asunto de la revista: METABOLISMO Año: 2014 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido