Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet.

Magnoni, Leonardo J; Crespo, Diego; Ibarz, Antoni; Blasco, Josefina; Fernández-Borràs, Jaume; Planas, Josep V

Magnoni, Leonardo J; Crespo, Diego; Ibarz, Antoni; Blasco, Josefina; Fernández-Borràs, Jaume; Planas, Josep V.

Afiliación

Magnoni LJ; Departament de Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Spain; Institut de Biomedicina de la Universitat de Barcelona (IBUB), Barcelona, Spain.

Comp Biochem Physiol A Mol Integr Physiol ; 166(3): 510-21, 2013 Nov.

Article en En | MEDLINE | ID: mdl-23968867

RESUMEN

Training at sustainable swimming speeds can produce changes in fish skeletal muscle that are important for aquaculture due to their growth-potentiating effects. Such changes may be even more relevant when fish are fed diets containing an increasing proportion of carbohydrates as an energy source. We evaluated the effects of moderate-intensity sustained swimming on the transcriptomic response of red and white muscle in rainbow trout fed a carbohydrate-rich diet using microarray and qPCR. Analysis of the red and white muscle transcriptome in resting or swimming (1.3 body lengths/s) fish for 30days revealed significant changes in the expression of a large number of genes (395 and 597, respectively), with a total of 218 differentially expressed genes (DEGs) common for both muscles. A large number of the genes involved in glucose use and energy generation, contraction, development, synthesis and catabolism of proteins were up-regulated in red and white muscle. Additionally, DEGs in both muscles were involved in processes of defense response and apoptosis. Skeletal muscle contraction activates a transcriptional program required for the successful adaptation of both muscles to the changing demands imposed by swimming conditions. Future studies should further clarify the mechanisms involved in the adaptation of both tissues to exercise and assess possible benefits of such conditions for cultured fish.

Asunto(s)

Carbohidratos de la Dieta/metabolismo; Fibras Musculares de Contracción Rápida/metabolismo; Fibras Musculares de Contracción Lenta/metabolismo; Oncorhynchus mykiss/metabolismo; Transcriptoma; Animales; Metabolismo de los Hidratos de Carbono; Proteínas de Peces/genética; Proteínas de Peces/metabolismo; Regulación de la Expresión Génica; Proteínas Musculares/genética; Proteínas Musculares/metabolismo; Análisis de Secuencia por Matrices de Oligonucleótidos; Oncorhynchus mykiss/genética; Especificidad de Órganos; Esfuerzo Físico/fisiología; Reacción en Cadena en Tiempo Real de la Polimerasa; Natación/fisiología

Palabras clave

Differential gene expression; Fish skeletal muscle; GLUT4; Glucose utilization; Moderate-intensity endurance exercise; Transcriptional regulation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbohidratos de la Dieta / Oncorhynchus mykiss / Fibras Musculares de Contracción Lenta / Fibras Musculares de Contracción Rápida / Transcriptoma Límite: Animals Idioma: En Revista: Comp Biochem Physiol A Mol Integr Physiol Asunto de la revista: BIOLOGIA MOLECULAR / FISIOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos

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