Development of a Luminex assay for the detection of swine antibodies to non-structural proteins of foot-and-mouth disease virus.

Chen, Tsu-Han; Lee, Fan; Lin, Yeou-Liang; Pan, Chu-Hsiang; Shih, Chia-Ni; Lee, Ming-Chang; Tsai, Hsiang-Jung

Chen, Tsu-Han; Lee, Fan; Lin, Yeou-Liang; Pan, Chu-Hsiang; Shih, Chia-Ni; Lee, Ming-Chang; Tsai, Hsiang-Jung.

Afiliación

Chen TH; Animal Health Research Institute, 376, Chung-Cheng Road, Tamsui District, New Taipei City 25158, Taiwan; Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, 1, Section 4 Roosevelt Road, Taipei 10617, Taiwan.

J Immunol Methods ; 396(1-2): 87-95, 2013 Oct 31.

Article en En | MEDLINE | ID: mdl-23962586

RESUMEN

Foot-and mouth disease (FMD), swine vesicular disease (SVD), and vesicular stomatitis (VS) are highly contagious vesicular diseases of swine but are not easy to differentiate clinically. For the purpose of instant detecting of FMD and differentiating it from the other vesicular diseases, a Luminex assay was developed. Sera from 64 infected, 307 vaccinated, and 280 naïve pigs were tested by the Luminex assay. Diagnostic sensitivity of the assay was 100%. Diagnostic specificity of the assay was 98.7% in vaccinated pigs and 97.5% to 100% in naïve pigs. Agreement between the results from the Luminex assay and those from a 3ABC polypeptide blocking ELISA was 96.3% with kappa statistics of 0.92. The Luminex assay can detect the immune response to NSP-3ABC in swine as early as eight days post-infection. Moreover, all of the 15 vaccinated but unprotected pigs were all detected by the Luminex assay. The results indicated that the Luminex assay has potential with specificity in detecting antibodies to FMDV 3ABC NSP and in distinguishing FMDV-infected pigs from with either SVDV or VSV.

Asunto(s)

Anticuerpos Antivirales/sangre; Fiebre Aftosa/diagnóstico; Inmunoensayo/veterinaria; Enfermedades de los Porcinos/diagnóstico; Proteínas no Estructurales Virales/inmunología; Animales; Diagnóstico Diferencial; Fiebre Aftosa/inmunología; Virus de la Fiebre Aftosa/inmunología; Microesferas; Sensibilidad y Especificidad; Porcinos; Enfermedades de los Porcinos/inmunología; Enfermedad Vesicular Porcina/diagnóstico; Vacunación/veterinaria; Estomatitis Vesicular/diagnóstico; Vacunas Virales

Palabras clave

Antibody detection; ELISA; Enzyme-linked immunosorbent assay; FMD; FMDV; Foot-and-mouth disease virus; HRP; Luminex assay; MFI; NC; NSP; Non-structural protein; OD; PC; PI; SP; SPF; SVDV; T/C; VSV; days post infection; dpi; enzyme-linked immunosorbent assay; foot-and-mouth disease; foot-and-mouth disease virus; horseradish peroxidase; median fluorescent intensity; multi-analyte profiling; negative control; non-structural protein; optical density; percentage of inhibition; positive control; specific-pathogen-free; structural protein; swine vesicular disease virus; test to control; vesicular stomatitis virus; xMAP

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Inmunoensayo / Proteínas no Estructurales Virales / Fiebre Aftosa / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Immunol Methods Año: 2013 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos

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