Protective effect of polypeptides from larva of housefly (Musca domestica) on hydrogen peroxide-induced oxidative damage in HepG2 cells.
Food Chem Toxicol
; 60: 385-90, 2013 Oct.
Article
en En
| MEDLINE
| ID: mdl-23933357
Housefly (Musca domestica) is an important medical insect and its larva is an ideal high protein food source. We isolated from housefly larvae the polypeptides hydrolyzed by neutral protease (PHNP), and investigated the protective effect of PHNP on hydrogen peroxide (H2O2)-induced oxidative damage in HepG2 cells. Cells exposed to H2O2 showed a marked decrease in proliferation and intracellular superoxide dismutase (SOD) activity, and a significant increase in reactive oxygen species (ROS) level and malondialdehyde (MDA) content. H2O2 also caused apoptosis and mitochondrial dysfunction including mitochondrial fragmentation and the loss of mitochondrial membrane potential. Pretreatment with PHNP at concentrations of 2.5, 5, 10 µg/mL blocked these H2O2-induced cellular events in a dose-dependent manner. The effect of PHNP at 10 µg/mL is equal to that of ascorbic acid at 10 µM. In summary, PHNP has a protective effect against H2O2-induced oxidative injury in cells due to its ability to decrease intracellular ROS and elevate antioxidant enzyme activities.
Palabras clave
2',7'-dichlorofluorescein; 2',7'-dichlorofluorescein-diacetate; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Antioxidant activity; Apoptosis; BSA; DCF; DCFH-DA; DMEM; Dulbecco's modified Eagle's medium; FITC; Housefly larva; MDA; MMP; MTT; Oxidative damage; PBS; PBST; PEF; PHNP; PI; ROS; Reactive oxygen species; Rh123; SOD; TBA; TRITC; VC; Vitamin C; WST-1; bovine serum albumin; fluorescein isothiocyanate; malondialdehyde; mitochondrial membrane potential; phosphate buffered solution; phosphate buffered solution/Tween 20; polypeptides hydrolyzed by neutral protease; propidium iodide; protein-enriched fraction; reactive oxygen species; rhodamine 123; superoxide dismutase; tetraethyl rhodamine isothiocyanate; thiobarbituric acid; water-soluble tetrazolium salt (sodium salt of 4-[3-(4iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate)
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Estrés Oxidativo
/
Moscas Domésticas
/
Peróxido de Hidrógeno
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Food Chem Toxicol
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Reino Unido