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Increased expression of glial fibrillary acidic protein in prefrontal cortex in psychotic illness.
Feresten, Abigail H; Barakauskas, Vilte; Ypsilanti, Athena; Barr, Alasdair M; Beasley, Clare L.
Afiliación
  • Feresten AH; Department of Psychiatry, University of British Columbia, Vancouver, Canada.
Schizophr Res ; 150(1): 252-7, 2013 Oct.
Article en En | MEDLINE | ID: mdl-23911257
Astrocyte dysregulation has been implicated in the pathophysiology of schizophrenia (SCZ) and bipolar disorder (BPD), however the exact nature of astrocytic alterations remains to be identified. In this study we investigated whether levels of four astrocyte-specific proteins; glial fibrillary acidic protein (GFAP), aldehyde dehydrogenase 1L1 (ALDH1L1), vimentin and excitatory amino acid transporter 1 (EAAT1) are altered in SCZ and BPD. Relative concentrations of GFAP, ALDH1L1, vimentin and EAAT1 were assessed post-mortem in dorsolateral prefrontal cortex in SCZ (n=35), BPD (n=34) and non-psychiatric controls (n=35) by western blotting. The same proteins were also quantified in cingulate cortex of rats administered the antipsychotics haloperidol and clozapine. Elevated levels of GFAP were observed in SCZ and BPD, when compared to controls. GFAP was also significantly increased when comparing individuals with psychotic symptoms against those without. Vimentin, ALDH1L1 and EAAT1 levels did not differ between groups. Rats exposed to antipsychotics did not exhibit significant differences in any astrocytic protein, suggesting that increased GFAP in SCZ is not attributable to antipsychotic treatment. Our findings indicate that astrocyte pathology may be associated with psychotic symptoms. Lack of ALDH1L1 and vimentin variability and increased GFAP levels may imply that astrocyte numbers are unchanged but astrocytes are partially activated, or may indicate a specific dysregulation of GFAP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trastornos Psicóticos / Regulación de la Expresión Génica / Corteza Prefrontal / Proteína Ácida Fibrilar de la Glía Tipo de estudio: Prognostic_studies Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Schizophr Res Asunto de la revista: PSIQUIATRIA Año: 2013 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trastornos Psicóticos / Regulación de la Expresión Génica / Corteza Prefrontal / Proteína Ácida Fibrilar de la Glía Tipo de estudio: Prognostic_studies Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Schizophr Res Asunto de la revista: PSIQUIATRIA Año: 2013 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos