[Effect of flavonoids of polygoni orientails fructus on human hepatoma cell line SMMC-7721].
Zhong Yao Cai
; 36(2): 255-9, 2013 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-23901654
OBJECTIVE: To investigate the inhibitory effects of flavonoids of Polygoni Orientails Fructus on cell cycle and apoptosis of human hepatoma cell line SMMC-7721. METHODS: MTT method was used to study the inhibitory rate and time-dose relationship of flavonoids on SMMC-7721. Flow cytometry PI staining and Annexin V-EGFP/PI double staining were used to measure the DNA concentration and apoptosis rate in SMMC-7721. RESULTS: Flavonoids of Polygoni Orientails Fructus had obvious inhibitory effect and with good linear relationship between time and dose. Flavonoids of Polygoni Orientails Fructus could regulate the G1/S transition in hepatoma cell, which could make the cell in S phase arrest, result in the accumulation of cells in S phase, blocked DNA synthesis and replication of cells, blocked tumor cells into the G2/M phase, so as to restrain the proliferation of tumor cells and induce the apoptosis of SMMC-7721 cells. And this inductive effect showed obvious time-dose-effect relationship. CONCLUSION: The flavonoids of Polygoni Orientails Fructus could inhibit the hepatoma cell line SMMC-7721 in vitro by inhibiting cell proliferation and inducing apoptosis of the cell in a time-dose dependence maner.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Flavonoides
/
Apoptosis
/
Polygonaceae
/
Proliferación Celular
/
Neoplasias Hepáticas
/
Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
Zh
Revista:
Zhong Yao Cai
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China