Staphylococcus aureus Penicillin-Binding Protein 2 Can Use Depsi-Lipid II Derived from Vancomycin-Resistant Strains for Cell Wall Synthesis.
Chemistry
; 19(36): 12104-12, 2013 Sep 02.
Article
en En
| MEDLINE
| ID: mdl-23873669
Vancomycin-resistant Staphylococcus aureus (S. aureus) (VRSA) uses depsipeptide-containing modified cell-wall precursors for the biosynthesis of peptidoglycan. Transglycosylase is responsible for the polymerization of the peptidoglycan, and the penicillin-binding protein 2 (PBP2) plays a major role in the polymerization among several transglycosylases of wild-type S. aureus. However, it is unclear whether VRSA processes the depsipeptide-containing peptidoglycan precursor by using PBP2. Here, we describe the total synthesis of depsi-lipid I, a cell-wall precursor of VRSA. By using this chemistry, we prepared a depsi-lipid II analogue as substrate for a cell-free transglycosylation system. The reconstituted system revealed that the PBP2 of S. aureus is able to process a depsi-lipid II intermediate as efficiently as its normal substrate. Moreover, the system was successfully used to demonstrate the difference in the mode of action of the two antibiotics moenomycin and vancomycin.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligosacáridos
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Staphylococcus aureus
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Muramoilpentapéptido Carboxipeptidasa
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Peptidoglicano
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Vancomicina
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Pared Celular
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Depsipéptidos
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Proteínas de Unión a las Penicilinas
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Staphylococcus aureus Resistente a Meticilina
/
Antibacterianos
Idioma:
En
Revista:
Chemistry
Asunto de la revista:
QUIMICA
Año:
2013
Tipo del documento:
Article
Pais de publicación:
Alemania