An electrochemiluminescence assay for the detection of bio threat agents in selected food matrices and in the screening of Clostridium botulinum outbreak strains associated with type A botulism.
J Sci Food Agric
; 94(4): 707-12, 2014 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-23873138
BACKGROUND: Specific screening methods for complex food matrices are needed that enable unambiguous and sensitive detection of bio threat agents (BTAs) such as Bacillus anthracis spores and microbial toxins (e.g. staphylococcal enterotoxin B (SEB) and clostridial botulinum neurotoxins (BoNTs)). The present study describes an image-based 96-well Meso Scale Discovery (MSD) electrochemiluminescence (ECL) assay for simultaneous detection of BTAs in dairy milk products. RESULTS: The limit of detection of this ECL assay is 40 pg mL⻹ for BoNT/A complex, 10 pg mL⻹ for SEB and 40000 CFU mL⻹ for Bacillus anthracis spores in dairy milk products. The ECL assay was successfully applied to screen type A Clostridium botulinum outbreak strains. CONCLUSION: The results of the study indicate that this ECL assay is very sensitive, rapid (<6 h) and multiplex in nature. The ECL assay has potential for use as an in vitro screening method for BTAs over other comparable immunoassays.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Toxinas Bacterianas
/
Contaminación de Alimentos
/
Inspección de Alimentos
/
Productos Lácteos
/
Clostridium botulinum tipo A
/
Luminiscencia
/
Enfermedades Transmitidas por los Alimentos
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Risk_factors_studies
/
Screening_studies
País/Región como asunto:
America do norte
Idioma:
En
Revista:
J Sci Food Agric
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido