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Transglutaminase-mediated macromolecular assembly: production of conjugates for food and pharmaceutical applications.
Mariniello, L; Porta, R; Sorrentino, A; Giosafatto, C V L; Rossi Marquez, G; Esposito, M; Di Pierro, P.
Afiliación
  • Mariniello L; Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario di Monte S. Angelo, Via Cinthia, 80126, Naples, Italy.
Amino Acids ; 46(3): 767-76, 2014 Mar.
Article en En | MEDLINE | ID: mdl-23860849
Various strategies have been explored in the last 20 years to modify the functional properties of proteins and, among these, protein/polymer conjugation resulted one of the most successful approaches. Thus, the surface modification of polypeptides of potential industrial interest by covalent attachment of different macromolecules is nowadays regarded as an extremely valuable technique to manipulate protein activities. Protein derivatives with a number of either natural or synthetic polymers, like different polysaccharides or polyethylene glycol, have been obtained by both chemical and enzymatic treatments, and in this context, the crosslinking enzyme transglutaminase is attracting an increasing attention as a simple and safe means for protein processing in vitro. In this short review, we summarized the most significant experimental findings demonstrating that a microbial form of the enzyme is an effective tool to obtain several biopolymer-based conjugates potentially useful for both food and pharmaceutical applications.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preparaciones Farmacéuticas / Transglutaminasas / Sustancias Macromoleculares / Alimentos Idioma: En Revista: Amino Acids Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Austria

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preparaciones Farmacéuticas / Transglutaminasas / Sustancias Macromoleculares / Alimentos Idioma: En Revista: Amino Acids Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Austria