Plasmid-encoded ComI inhibits competence in the ancestral 3610 strain of Bacillus subtilis.
J Bacteriol
; 195(18): 4085-93, 2013 Sep.
Article
en En
| MEDLINE
| ID: mdl-23836866
Natural competence is a process by which bacteria construct a membrane-associated machine for the uptake and integration of exogenous DNA. Many bacteria harbor genes for the DNA uptake machinery and yet are recalcitrant to DNA uptake for unknown reasons. For example, domesticated laboratory strains of Bacillus subtilis are renowned for high-frequency natural transformation, but the ancestral B. subtilis strain NCIB3610 is poorly competent. Here we find that endogenous plasmid pBS32 encodes a small protein, ComI, that inhibits transformation in the 3610 strain. ComI is a single-pass trans-membrane protein that appears to functionally inhibit the competence DNA uptake machinery. Functional inhibition of transformation may be common, and abolishing such inhibitors could be the key to permitting convenient genetic manipulation of a variety of industrially and medically relevant bacteria.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
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Bacillus subtilis
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Proteínas Bacterianas
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Transformación Bacteriana
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Competencia de la Transformación por ADN
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Proteínas de la Membrana
Idioma:
En
Revista:
J Bacteriol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos