Simultaneous species-specific PCR detection and viability testing of poly(vinyl alcohol) cryogel-entrapped Rhodococcus spp. after their exposure to petroleum hydrocarbons.

Kuyukina, Maria S; Ivshina, Irena B; Serebrennikova, Marina K; Rubtsova, Ekaterina V; Krivoruchko, Anastasiya V

Kuyukina, Maria S; Ivshina, Irena B; Serebrennikova, Marina K; Rubtsova, Ekaterina V; Krivoruchko, Anastasiya V.

Afiliación

Kuyukina MS; Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, 13 Golev Street, 614081 Perm, Russia; Microbiology and Immunology Department, Perm State National Research University, 15 Bukirev Street, 614900 Perm, Russia. Electronic address: kuyukina@iegm.ru.
Ivshina IB; Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, 13 Golev Street, 614081 Perm, Russia; Microbiology and Immunology Department, Perm State National Research University, 15 Bukirev Street, 614900 Perm, Russia.
Serebrennikova MK; Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, 13 Golev Street, 614081 Perm, Russia.
Rubtsova EV; Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, 13 Golev Street, 614081 Perm, Russia; Microbiology and Immunology Department, Perm State National Research University, 15 Bukirev Street, 614900 Perm, Russia.
Krivoruchko AV; Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, 13 Golev Street, 614081 Perm, Russia; Microbiology and Immunology Department, Perm State National Research University, 15 Bukirev Street, 614900 Perm, Russia.

J Microbiol Methods ; 94(2): 135-140, 2013 Aug.

Article en En | MEDLINE | ID: mdl-23747656

Asunto(s)

Hidrocarburos/farmacología; Petróleo/metabolismo; Reacción en Cadena de la Polimerasa/métodos; Rhodococcus/crecimiento & desarrollo; Rhodococcus/aislamiento & purificación; Células Inmovilizadas/química; Células Inmovilizadas/clasificación; Células Inmovilizadas/efectos de los fármacos; Hidrocarburos/metabolismo; Viabilidad Microbiana/efectos de los fármacos; Alcohol Polivinílico/química; Rhodococcus/efectos de los fármacos; Rhodococcus/genética; Especificidad de la Especie

Palabras clave

16S rDNA-targeted PCR; Cryogel-immobilized bacteria; Petroleum hydrocarbons; Respirometry; Rhodococcus; Viability testing

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodococcus / Petróleo / Reacción en Cadena de la Polimerasa / Hidrocarburos Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: J Microbiol Methods Año: 2013 Tipo del documento: Article Pais de publicación: Países Bajos

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