[Peptides analysis in digested edible bird's nest by HPLC-MS].
Zhongguo Zhong Yao Za Zhi
; 38(5): 714-9, 2013 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-23724682
Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Espectrometría de Masas
/
Aves
/
Proteolisis
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China