[Use of 2-DE and MALDI-TOF-MS to screen differentially expressed serum proteins in patients with oral lichen planus before and after hydroxychloroquine treatment].
Shanghai Kou Qiang Yi Xue
; 22(2): 184-8, 2013 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-23708033
PURPOSE: To establish 2-dimensional gel electrophoresis images and compare the differences of serum proteins of oral lichen planus patients before and after hydroxychloroquine therapy. METHODS: The serum of oral lichen planus patients before and after hydroxychloroquine therapy were collected, and total protein were extracted. Differential proteome profiles were established and analysed by means of 2-DE and MALDI-TOF-MS. The types and functions of protein were analyzed. SAS 9.12 software package was used for statistical analysis. RESULTS: Six proteins were well characterized including plasminogen precursor,Apo A-IV precursor, C4A/C4B complement, C2 precursor, Vitamin D binding protein and hypothetical protein. The differences were statistically significant. CONCLUSIONS: Plasminogen precursor, Apo A-IV precursor, C4A/C4B complement, C2 precursor, Vitamin D binding protein and hypothetical protein are differentially expressed in oral lichen planus patients before and after hydroxychloroquine therapy, but the results need to be validated by other biochemical technologies.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Liquen Plano Oral
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Límite:
Humans
Idioma:
Zh
Revista:
Shanghai Kou Qiang Yi Xue
Asunto de la revista:
ODONTOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China