Delayed effects of neonatal exposure to 17alpha-ethynylestradiol on the estrous cycle and uterine carcinogenesis in Wistar Hannover GALAS rats.

Takahashi, Miwa; Inoue, Kaoru; Morikawa, Tomomi; Matsuo, Saori; Hayashi, Seigo; Tamura, Kei; Watanabe, Gen; Taya, Kazuyoshi; Yoshida, Midori

Takahashi, Miwa; Inoue, Kaoru; Morikawa, Tomomi; Matsuo, Saori; Hayashi, Seigo; Tamura, Kei; Watanabe, Gen; Taya, Kazuyoshi; Yoshida, Midori.

Afiliación

Takahashi M; Division of Pathology, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan. mtakahashi@nihs.go.jp

Reprod Toxicol ; 40: 16-23, 2013 Sep.

Article en En | MEDLINE | ID: mdl-23707403

RESUMEN

We investigated the delayed effects of neonatal exposure to 17α-ethynylestradiol (EE) on the female reproductive tract using Wistar Hannover GALAS rats. Female pups received single injections of EE (0, 0.02, 0.2, 2, 20, or 200 µg/kg) within 24h after birth and estrous cyclicity was observed until 10 months of age. All animals were treated at 9 weeks of age with the uterine carcinogen, N-ethyl-N'-nitro-N-nitrosoguanidine. Although the vaginal opening was not affected, abnormal cycles were significantly increased from 0.2 µg/kg. Persistent estrus was prominent and the incidence increased age- and dose-dependently. Severity of atypical hyperplasia of the uterus tended to increase from 2 µg/kg. In these groups, serum progesterone level was lowered relative to estradiol level. In conclusion, estrous cyclicity was a sensitive indicator reflecting delayed effects on the female reproductive tract. Early onset of anovulation leading to prolonged estrogen exposure might be a risk factor for uterine carcinogenesis.

Asunto(s)

Estrógenos/toxicidad; Ciclo Estral/efectos de los fármacos; Etinilestradiol/toxicidad; Glándulas Mamarias Animales/efectos de los fármacos; Efectos Tardíos de la Exposición Prenatal; Útero/efectos de los fármacos; Adenocarcinoma/sangre; Adenocarcinoma/inducido químicamente; Adenocarcinoma/patología; Animales; Anovulación/sangre; Anovulación/inducido químicamente; Anovulación/patología; Carcinógenos; Estradiol/sangre; Femenino; Hiperplasia/sangre; Hiperplasia/inducido químicamente; Hiperplasia/patología; Glándulas Mamarias Animales/patología; Metilnitronitrosoguanidina/análogos & derivados; Tamaño de los Órganos/efectos de los fármacos; Embarazo; Progesterona/sangre; Ratas; Ratas Wistar; Neoplasias Uterinas/sangre; Neoplasias Uterinas/inducido químicamente; Neoplasias Uterinas/patología; Útero/patología; Vagina/efectos de los fármacos; Vagina/crecimiento & desarrollo

Palabras clave

17Alpha-ethynylestradiol; 17α-ethynylestradiol; DES; Delayed effects; E2; EE; ENNG; Estrous cycle; FSH; HPG; LH; N-ethyl-N'-nitro-N-nitrosoguanidine; Neonatal exposure; P4; PND; PRL; Uterine carcinogenesis; diethylstilbestrol; estradiol-17ß; follicle-stimulating hormone; hypothalamus-pituitary-gonadal; lutenizing hormone; postnatal day; progesterone; prolactin

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Efectos Tardíos de la Exposición Prenatal / Útero / Ciclo Estral / Estrógenos / Etinilestradiol / Glándulas Mamarias Animales Tipo de estudio: Risk_factors_studies Límite: Animals / Pregnancy Idioma: En Revista: Reprod Toxicol Asunto de la revista: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Año: 2013 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google