TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants.
Plant Mol Biol
; 83(3): 279-85, 2013 Oct.
Article
en En
| MEDLINE
| ID: mdl-23689819
Transcription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of organisms. The primary advantage of TALENs over other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their ease of assembly, reliability of function, and their broad targeting range. Here we report the assembly of several TALENs for a specific genomic locus in barley. The cleavage activity of individual TALENs was first tested in vivo using a yeast-based, single-strand annealing assay. The most efficient TALEN was then selected for barley transformation. Analysis of the resulting transformants showed that TALEN-induced double strand breaks led to the introduction of short deletions at the target site. Additional analysis revealed that each barley transformant contained a range of different mutations, indicating that mutations occurred independently in different cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Transformación Genética
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Hordeum
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Genoma de Planta
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Endonucleasas
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Mutación
Idioma:
En
Revista:
Plant Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
2013
Tipo del documento:
Article
Pais de publicación:
Países Bajos