A sensitive and specific PCR assay for the detection of Baylisascaris schroederi eggs in giant panda feces.
Parasitol Int
; 62(5): 435-6, 2013 Oct.
Article
en En
| MEDLINE
| ID: mdl-23688803
Baylisascaris schroederi is one of the most common intestinal nematodes in giant pandas. It can cause severe baylisascariasis which is highly infectious in its natural hosts. A rapid and reliable diagnosis of parasite infections is crucial to protect giant pandas, as well as for environmental monitoring and disease surveillance. Here, we established a specific PCR assay for B. schroederi detection which was targeting a 331-bp long fragment of the mitochondrial cytochrome c oxidase subunit II (COII) gene. Fifty fresh fecal samples collected from captive giant pandas were tested by the established PCR assay and the traditional flotation technique. DNA extracted from a single B. schroederi egg could be successfully amplified, while no cross-reactivity was found with DNA from Ancylostoma caninum eggs. The detection rate of the PCR assay was 68%, which was higher than that of the traditional egg flotation (46%). Our findings demonstrated that the PCR assay is sensitive and specific for the detection and identification of B. schroederi eggs. Therefore, it could become a useful tool for the investigation of B. schroederi infections in giant pandas.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ursidae
/
Ascaridoidea
/
Reacción en Cadena de la Polimerasa
/
Infecciones por Ascaridida
/
Heces
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Parasitol Int
Asunto de la revista:
PARASITOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos